Characterization of Thermal and Physical Properties of Biofield Treated Acrylamide and 2-Chloroacetamide

Acrylamide (AM) and 2-chloroacetamide (CA) are widely used in diverse applications such as biomedical, drug delivery, waste water treatment, and heavy metal ion removal. The objective of this study was to evaluate the influence of biofield treatment on physical and thermal properties of amide group containing compounds (AM and CA). The study was performed in two groups (control and treated). The control group remained as untreated, and biofield treatment was given to treated group. The control and treated compounds were characterized by X-ray diffraction (XRD), differential scanning calorimetry (DSC), thermogravimetric analysis (TGA) and surface area analysis. XRD of treated AM showed decrease in intensity of peaks as compared to control sample. However, the treated AM showed increase in volume of unit cell (0.16%) and molecular weight (0.16%) as compared to control. The crystallite size was decreased by 33.34% in treated AM as compared to control Whereas, the XRD diffractogram of treated CA showed increase in intensity of crystalline peaks as compared to control. The percentage volume of unit cell (-1.92%) and molecular weight (-1.92%) of treated CA were decreased as compared to control. However, significant increase in crystallite size (129.79%) was observed in treated CA as compared to control. DSC of treated AM showed increase in melting temperature as compared to control sample. Similarly, the treated CA also showed increase in melting temperature with respect to control. Latent heat of fusion (ΔH) was significantly changed in treated AM and CA as compared to control samples. TGA showed increase in thermal stability of treated AM and CA which was evidenced by increase in thermal decomposition temperature (Tmax) as compared to control. Surface area analysis of treated AM showed increase (31.6%) in surface area as compared to control. However, a decrease (30.9%) in surface area was noticed in treated CA as compared to control. Study results suggest that biofield treatment has significant impact on the physical and thermal properties of AM and CA.


Introduction
Acrylamide (AM) is a monomer used for synthesis of polyacrylamide that is commonly utilized for biomedical, pharmaceutical and wastewater treatment applications. AM based polymer was introduced as a support matrix for electrophoresis in 1959 [1]. Generally, the AM based gels are produced using bifunctional cross linker such as N-methylene bisacrylamide [2]. AM based polymers are widely used in areas such as enzyme immobilization [3,4], carrier for delivery of drugs and bioactive compounds [5][6][7][8][9], stimuli responsive materials [10,11] and in non-absorbable soft tissue fillers used for body contouring in reconstructive surgery [12]. On the other hand 2-chloroacetamide (CA) is a chlorinated organic compound used as herbicide and preservative [13]. The amide group of CA has better selectivity towards mercury binding, hence it has been commonly used for heavy metal ion removal applications [14]. The structural and conformational nature of CA have been previously studied by many researchers [15,16]. However, the AM and CA have toxicity problems which need to be addressed in order to make it useful for other areas such as biomedical and novel drug development. Biofield treatment has been recently used as a strategy for changing the atomic and physical properties of various materials [17][18][19][20].
Bioelectrography dates back to 1770 when German scientist George Christopher Lichtenberg observed light coming out from subjects in electrical fields [21]. Researchers have showed that short lived electrical events or action potential exist in several type of mammalian cells such as neurons, muscle cells, and endocrine cells [22]. For instance the cells present in central nervous system of human body communicate with each another by means of electrical signals that travel along the nerve processes. Therefore, it was hypothesized that biofield exists around the human body and the evidence was found using Electromyography, Electrocardiography and electroencephalogram [23]. Human has the ability to harness the energy from environment or universe and can transmit into any living or nonliving object around the Globe. The object(s) always receive the energy and responds in a useful way that is called biofield energy and the process is known as biofield treatment. Mr. Mahendra K. Trivedi is known to transform the characteristics of various living and non-living things using his biofield treatment. The biofield treatment had significantly improved the production and quality of various agricultural products [24][25][26][27]. Biofield treatment has shown excellent results in improving antimicrobial susceptibility, and alteration of biochemical reactions, as well as induced alterations in characteristics of pathogenic microbes [28][29][30]. The biofield treatment had also caused an increase in growth and anatomical characteristics Abstract Acrylamide (AM) and 2-chloroacetamide (CA) are widely used in diverse applications such as biomedical, drug delivery, waste water treatment, and heavy metal ion removal. The objective of this study was to evaluate the influence of biofield treatment on physical and thermal properties of amide group containing compounds (AM and CA). The study was performed in two groups (control and treated). The control group remained as untreated, and biofield treatment was given to treated group. The control and treated compounds were characterized by X-ray diffraction (XRD), differential scanning calorimetry (DSC), thermogravimetric analysis (TGA) and surface area analysis. XRD of treated AM showed decrease in intensity of peaks as compared to control sample. However, the treated AM showed increase in volume of unit cell (0.16%) and molecular weight (0.16%) as compared to control. The crystallite size was decreased by 33.34% in treated AM as compared to control Whereas, the XRD diffractogram of treated CA showed increase in intensity of crystalline peaks as compared to control. The percentage volume of unit cell (-1.92%) and molecular weight (-1.92%) of treated CA were decreased as compared to control. However, significant increase in crystallite size (129.79%) was observed in treated CA as compared to control. DSC of treated AM showed increase in melting temperature as compared to control sample. Similarly, the treated CA also showed increase in melting temperature with respect to control. Latent heat of fusion (∆H) was significantly changed in treated AM and CA as compared to control samples. TGA showed increase in thermal stability of treated AM and CA which was evidenced by increase in thermal decomposition temperature (T max ) as compared to control. Surface area analysis of treated AM showed increase (31.6%) in surface area as compared to control. However, a decrease (30.9%) in surface area was noticed in treated CA as compared to control. Study results suggest that biofield treatment has significant impact on the physical and thermal properties of AM and CA. of an herb Pogostemon cablin that is commonly used in perfumes, in incense/insect repellents, and alternative medicine [31]. By conceiving the above research outcome of biofield treatment, an attempt was made here to study the influence of biofield on physical and thermal properties of AM and CA.

Materials and Methods
Acrylamide (AM) and 2-chloroacetamide (CA) were procured from S D Fine Chemicals Pvt., Ltd., India. Each sample was divided into two parts; one was kept as a control, while other was subjected to Mr. Trivedi's biofield treatment and coded as treated sample. The treatment group in sealed pack was handed over to Mr. Trivedi for biofield treatment under standard laboratory condition. Mr. Trivedi provided the treatment through his energy transmission process to the treated group without touching the sample. The biofield treated samples were returned in the similar sealed condition for characterization using XRD, DSC, TGA and surface area analysis techniques. Characterization X-ray diffraction (XRD) study: XRD analysis was carried out on Phillips, Holland PW 1710 X-ray diffractometer system, which had a copper anode with nickel filter. The radiation of wavelength used by the XRD system was 1.54056 Å. The data obtained from this XRD were in the form of a chart of 2θ vs. intensity and a detailed table containing peak intensity counts, d value (Å), peak width (θ°), relative intensity (%) etc. Additionally, PowderX software was used to calculate unit cell volume.
The crystallite size (G) was calculated by using formula: Here, λ is the wavelength of radiation used and k is the equipment constant (=0.94). However, the percentage change in all parameters such as, unit cell volume and percent change in crystallite size was calculated using the following equation: Where, V c and V t are the unit cell volume of control and treated powder samples respectively Where, G c and G t are crystallite size of control and treated powder samples respectively.
The molecular weight of atom was calculated using following equation: Molecular weight=number of protons × weight of a proton + number of neutrons x weight of a neutron + number of electrons x weight of an electron.
Molecular weight in g/Mol was calculated from the weights of all atoms in a molecule multiplied by the Avogadro number (6.023×10 23 ). The percent change in molecular weight was calculated using the following equation: Where, M c and M t are molecular weight of control and treated powder sample respectively. Differential scanning calorimetry (DSC) study: The control and treated samples (AM and CA) were analyzed by using a Pyris-6 Perkin Elmer DSC on a heating rate of 10°C/min under air atmosphere and air was flushed at flow rate of 5 mL/min. Thermogravimetric analysis-differential thermal analysis (TGA-DTA): Thermal stability of control and treated samples (AM and CA) were analyzed by using Metller Toledo simultaneous TGA and Differential thermal analyzer (DTA). The samples were heated from room temperature to 400°C with a heating rate of 5°C/min under air atmosphere.
Surface area analysis: Surface area of AM and CA were characterized by surface area analyzer, SMART SORB 90 BET using ASTM D 5604 method which had a detection range of 0.2-1000 m 2 /g.

Results and Discussion
X-ray diffraction X-ray diffraction study was conducted to study the crystalline nature of the control and treated AM. XRD diffractogram of control AM showed (Figure 1) intense crystalline peaks at 2θ equals to 11.90°, 19.34°, 19.55°, 24.00°, 24.51°, 28.27°, 28.53°, 36.38° and 49.21°. These peaks showed the crystalline nature of AM. Whereas, the treated AM showed decreased intensity of the XRD peaks. The XRD diffractogram showed crystalline peaks at 2θ equals to 11.75°, 11.99°, 19.17°, 19.32°, 23.69°, 23.97°, 24.14°, 28.50° and 36.05°. The decrease in intensity of crystalline planes after biofield treatment of AM may be due to disturbed regular pattern of the atoms. The volume of unit cell, crystallite size and change in molecular weight were computed from the XRD diffractogram using Powder X software. The results are presented in Figure 2. An increase (0.16%) in volume of unit cell was observed in treated AM as compared to control. The treated AM showed 0.16% increase in molecular weight as compared to control. It is hypothesized that biofield energy possibly acted on treated AM crystals at nuclear level and altered the number of proton and neutrons as compared to control, which may led to increase the molecular weight. The treated AM (46.20 nm) showed (Table 1) decrease in crystallite size as compared to control (69.31 nm) and percentage decrease in crystallite size was 33.34% ( Figure 3). The existence of severe lattice strains was evidenced by the change in unit cell volume. Thus, it is assumed that presence of these internal strains might be a reason for fracturing the grains into sub grains which leads to decrease in crystallite size of treated sample. The XRD diffractogram of control and treated CA are presented in  35.63° and 44.78°. The significant increase in intensity of the XRD peaks revealed the enhanced crystalline nature of the sample. It is presumed that biofield energy may be absorbed by the CA crystals, which led to formation of more symmetrical crystalline long-range pattern; that caused increase in crystallinity. The volume of unit cell of treated CA was decreased with respect to control sample and the percentage decrease in volume of unit cell was -1.92%. The molecular weight of treated CA was reduced by 1.92% as compared to control and this may be due to decrease in number of protons and neutrons after biofield treatment (Figure 2). However, the treated CA showed ( Table  1) a significant increase in crystallite size (108.60 nm) as compared to control CA (47.26 nm). The percentage change in crystallite size was 129.79%. This showed ( Figure 3) the significant impact of biofield treatment on increasing the crystallite size of treated CA with respect to control. It was previously reported that crystallite size increases with elevation in temperature or thermal energy. Hence, it is assumed that biofield energy may reduce the thermodynamically driving force which automatically causes decrease in nucleus densities and raises the crystallite size [32,33].

DSC studies
The DSC thermogram of control and treated AM are shown in Figure 5. The DSC thermogram of control AM showed the presence of sharp endothermic peak at 86°C which was due to melting temperature of the control. However, the treated AM showed ( Figure 5) an increase in melting temperature peak (88°C) as compared to control. It indicated that biofield treated AM had better thermal stability as compared to control. DSC thermogram of control and treated CA are depicted in Figure 6. The DSC graph of the control sample showed sharp endothermic inflexion at 114°C which was responsible for melting of the compound. DSC thermogram of control CA showed another two endothermic peaks at 135°C and 168°C. Katayama performed X-ray investigation on CA and found that there are two forms of compound in crystalline state, one being more stable than other [34]. Hence, it is presumed that two endothermic peaks in control CA may be represented to two crystalline polymorphs present in the compound. Whereas, the treated CA showed ( Figure 6) an increase in melting temperature and it was observed at 116°C. It confirmed that biofield treatment may induced regular pattern in the CA atoms and hence increase in thermal stability. It was reported that γ radiation treatment increased the thermal stability of poly (3-hexadecythiophene). Therefore, it is presumed that biofield treatment may cause conformational changes and crosslinking in AM and CA compounds which led to increase in thermal stability [35]. Latent heat of fusion (∆H) was calculated from the DSC data and results are depicted in Table 2. The control AM showed a ∆H of 231.96 J/g and it was decreased to 156.83 J/g in treated

TGA studies
Thermal stability of the control and treated AM and CA compounds were evaluated using TGA. TGA thermogram of control and treated AM are presented in Figures 7 and 8. The TGA thermogram of control AM showed (Figure 7) one step thermal degradation pattern. The thermal degradation commence at around 148°C (onset) and degradation stopped at around 195°C (end set). This step showed a major weight loss and the sample lost 58.82% of its weight. However, DTA thermogram of control AM showed an endothermic peak at 86°C which may be associated with the melting temperature of the sample. The thermogram showed another endothermic peak at 174°C that may be due to thermal decomposition or breaking of intermolecular hydrogen bonding between AM. Derivative thermogravimetry (DTG) of the control AM exhibited the maximum thermal decomposition temperature (T max ) at 164°C. However, the treated AM thermogram also showed ( Figure 8) single step thermal decomposition pattern. The thermal degradation started at around 135°C (onset) and terminated at around 190°C (end set). During this step the treated AM showed 68.68% of weight loss. DTA thermogram of the treated AM showed melting peak at 85°C and second endothermic was seen at 176°C (thermal decomposition). The DTG thermogram of treated AM showed no change in T max value (164°C) as compared to control. It may be corroborated to no alteration in thermal stability of treated AM with respect to control after biofield treatment. The TGA thermogram of control and treated CA are shown in Figures 9 and 10. The control CA showed occurrence of one step thermal degradation pattern. Thermal degradation commenced at around 120°C (onset) and completed at around 190°C (end set). During this process the sample showed ( Figure 9) major weight loss (66.09%) that might be due to thermal decomposition of the CA chain. DTA thermogram of CA showed an endothermic peak at 115°C; associated with the melting of the sample. Another endothermic event was noticed at 162°C that may be due to thermal decomposition. The T max of control CA was observed at 150°C as shown by the DTG thermogram. TGA thermogram of treated CA showed ( Figure 10) one step thermal degradation between 130-210°C. During this thermal event sample showed rapid thermal degradation and weight loss (61.16%). However, the DTA thermogram of treated CA showed two endothermic peaks at 116°C and 176°C. The former endothermic was due to melting and later peak was attributed to thermal decomposition. The DTG thermogram of treated CA showed increase in T max value (167°C) as compared to control sample (150°C). This increase in thermal decomposition may be correlated to high thermal stability of the treated CA.

Surface area analysis
Surface area of AM and CA was investigated using BET method. The surface area result of control and treated compounds (AM and CA) are presented in Table 3. The control AM showed a surface area of 0.42 m 2 /g, however, the treated AM showed 0.55 m 2 /g. The percentage increase in surface area was 31.6% in the treated AM

Conclusion
The present work investigated the influence of biofield treatment on physical and thermal properties of AM and CA. The XRD results showed the decrease in crystallinity of treated AM as compared to control. The crystallite size was also decreased in treated AM with respect to control. Whereas, treated CA showed significant increase in crystallite size as compared to control. DSC showed increase in melting temperature of both treated AM and CA with respect to control. Additionally, latent heat of fusion of treated compounds was substantially changed as compared to control. TGA showed enhanced thermal decomposition temperature in CA as compared to control that indicated the higher thermal stability after biofield treatment. However, no change in thermal stability was observed in treated AM with respect to control. Overall, the results indicated that biofield treatment has significant impact in alteration of the physical and thermal properties of AM and CA.   Table 3: Surface area data of acrylamide (AM) and 2-chloroacetamide (CA).
sample as compared to control. It is hypothesized that energy milling provided by biofield treatment might cause fracturing in particles and thus reduction in particle size. Hence, reduction in particle size causes increase in surface area of the treated AM [36,37]. The control CA showed surface area 0.65 m 2 /g; however, it decreased to 0.45 m 2 /g in treated sample. The percentage decrease in surface area was 30.9% in treated CA as compared to control sample. The biofield energy may increase the particle size that lead to decrease in surface area.