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Tridimensional Visualization and Analysis of Early Human Development

Abstract : Generating a precise cellular and molecular cartography of the human embryo is essential to our understanding of the mechanisms of organogenesis in normal and pathological conditions. Here, we have combined whole-mount immunostaining, 3DISCO clearing, and light-sheet imaging to start building a 3D cellular map of the human development during the first trimester of gestation. We provide high-resolution 3D images of the developing peripheral nervous, muscular, vascular, cardiopulmonary, and urogenital systems. We found that the adult-like pattern of skin innervation is established before the end of the first trimester, showing important intra- and inter-individual variations in nerve branches. We also present evidence for a differential vascularization of the male and female genital tracts concomitant with sex determination. This work paves the way for a cellular and molecular reference atlas of human cells, which will be of paramount importance to understanding human development in health and disease.
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Submitted on : Friday, March 31, 2017 - 11:55:18 AM
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Morgane Belle, David Godefroy, Gérard Couly, Samuel A. Malone, Francis Collier, et al.. Tridimensional Visualization and Analysis of Early Human Development. Cell, Elsevier, 2017, 169 (1), pp.161-173.e12. ⟨10.1016/j.cell.2017.03.008⟩. ⟨hal-01497677⟩

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