Single-Step DNA Detection Assay Monitoring Dual-Color Light Scattering from Individual Metal Nanoparticle Aggregates

Abstract : Efficiently detecting DNA sequences within a limited time is vital for disease screening and public health monitoring. This calls for a new method that combines high sensitivity, fast read-out time and easy manipulation of the sample, avoiding the extensive steps of DNA amplification, purification or grafting to a surface. Here, we introduce photon cross correlation spectroscopy as a new method for specific DNA sensing with high sensitivity in a single step homogeneous solution phase. Our approach is based on confocal dual-color illumination and detection of the scattering intensities from individual silver nanoparticles and gold nanorods. In the absence of the target DNA, the nanoparticles move independently and their respective scattering signals are uncorrelated. In the presence of the target DNA, the probe-functionalized gold and silver nanoparticles assemble via DNA hybridization with the target, giving rise to temporal coincidences between the signals scattered by each nanoparticle. The degree of coincidence accurately quantifies the amount of target DNA. To demonstrate the efficiency of our technique, we detect a specific DNA sequence of sesame, an allergenic food ingredient, for a range of concentration from 5 pM to 1.5 nM with a limit of detection of 1 pM. Our method is sensitive and specific enough to detect single nucleotide deletion and mismatch. With the dual-color scattering signals being much brighter than fluorescence-based analogs, the analysis is fast, quantitative, and simple to operate, making it valuable for biosensing applications.
Complete list of metadatas

Cited literature [9 references]  Display  Hide  Download

https://hal.archives-ouvertes.fr/hal-01490247
Contributor : Jérome Wenger <>
Submitted on : Wednesday, June 7, 2017 - 2:17:29 PM
Last modification on : Monday, March 4, 2019 - 2:04:23 PM
Long-term archiving on : Friday, September 8, 2017 - 12:43:16 PM

File

Vial_acssensors2017.pdf
Publication funded by an institution

Identifiers

Collections

Citation

Stéphanie Vial, Youri Berrahal, Marta Prado, Jérôme Wenger. Single-Step DNA Detection Assay Monitoring Dual-Color Light Scattering from Individual Metal Nanoparticle Aggregates. ACS Sensors, American Chemical Society, 2017, 2 (2), pp.251-256. ⟨10.1021/acssensors.6b00737⟩. ⟨hal-01490247⟩

Share

Metrics

Record views

136

Files downloads

84