Skip to Main content Skip to Navigation
Journal articles

Impact of copper ligand mutations on a cupredoxin with a green copper center.

Abstract : Mononuclear cupredoxins contain a type 1 copper center with a trigonal or tetragonal geometry usually maintained by four ligands, a cystein, two histidines and a methionine. The recent discovery of new members of this family with unusual properties demonstrates, however, the versatility of this class of proteins. Changes in their ligand set lead to drastic variation in their metal site geometry and in the resulting spectroscopic and redox features. In our work, we report the identification of the copper ligands in the recently discovered cupredoxin AcoP. We show that even though AcoP possesses a classical copper ligand set, it has a highly perturbed copper center. In depth studies of mutant's properties suggest a high degree of constraint existing in the copper center of the wild type protein and even the addition of exogenous ligands does not lead to the reconstitution of the initial copper center. Not only the chemical nature of the axial ligand but also constraints brought by its covalent binding to the protein backbone might be critical to maintain a green copper site with high redox potential. This work illustrates the importance of experimentally dissecting the molecular diversity of cupredoxins to determine the molecular determinants responsible for their copper center geometry and redox potential.
Document type :
Journal articles
Complete list of metadatas

https://hal-amu.archives-ouvertes.fr/hal-01481398
Contributor : Laure Azzopardi <>
Submitted on : Wednesday, April 18, 2018 - 4:20:54 PM
Last modification on : Wednesday, May 27, 2020 - 4:02:05 AM

Links full text

Identifiers

Collections

Citation

Magali Roger, Giuliano Sciara, Frédéric Biaso, Elisabeth Lojou, Xie Wang, et al.. Impact of copper ligand mutations on a cupredoxin with a green copper center.. BBA - Biochimica et Biophysica Acta, Elsevier, 2017, 1858 (5), pp.351-359. ⟨10.1016/j.bbabio.2017.02.007⟩. ⟨hal-01481398⟩

Share

Metrics

Record views

144