Evidence for a genetic sex determination in Cnidaria, the Mediterranean red coral (Corallium rubrum)

Abstract : Sexual reproduction is widespread among eukaryotes, and the sex-determining processes vary greatly among species. While genetic sex determination (GSD) has been intensively described in bilaterian species, no example has yet been recorded among non-bilaterians. However, the quasi-ubiquitous repartition of GSD among multicellular species suggests that similar evolutionary forces can promote this system, and that these forces could occur also in non-bilaterians. Studying sex determination across the range of Metazoan diversity is indeed important to understand better the evolution of this mechanism and its lability. We tested the existence of sex-linked genes in the gonochoric red coral (Corallium rubrum, Cnidaria) using restriction site-associated DNA sequencing. We analysed 27 461 single nucleotide polymorphisms (SNPs) in 354 individuals from 12 populations including 53 that were morphologically sexed. We found a strong association between the allele frequencies of 472 SNPs and the sex of individuals, suggesting an XX/XY sex-determination system. This result was confirmed by the identification of 435 male-specific loci. An independent test confirmed that the amplification of these loci enabled us to identify males with absolute certainty. This is the first demonstration of a GSD system among non-bilaterian species and a new example of its convergence in multicellular eukaryotes.
Document type :
Journal articles
Complete list of metadatas

Cited literature [43 references]  Display  Hide  Download

https://hal.archives-ouvertes.fr/hal-01480527
Contributor : Anne Modat <>
Submitted on : Wednesday, March 1, 2017 - 2:22:46 PM
Last modification on : Wednesday, May 29, 2019 - 11:40:04 AM
Long-term archiving on : Tuesday, May 30, 2017 - 5:18:05 PM

File

Pratlong-2017-RSocOpenSci-Evid...
Publisher files allowed on an open archive

Identifiers

Citation

Marine Pratlong, Anne Haguenauer, Sandrine Chenesseau, P Brener, P Mitta, et al.. Evidence for a genetic sex determination in Cnidaria, the Mediterranean red coral (Corallium rubrum). rsos.royalsocietypublishing.org on, 2017, 4, pp.160880. ⟨http://rsos.royalsocietypublishing.org/content/4/3/160880⟩. ⟨10.1098/rsos.160880⟩. ⟨hal-01480527⟩

Share

Metrics

Record views

707

Files downloads

146