International standardized procedures forin vivo evaluation of multi-walled carbon nanotube toxicity in water

Florence Mouchet (1, 2) , Christian Gancet (2, 3) , Emmanuel Flahaut (2, 4) , Eric Pinelli (1, 2) , Jean–charles Boutonnet (2, 5) , Laury Gauthier (1, 2)

1 LEFE - Laboratoire Ecologie Fonctionnelle et Environnement
2 NAnotUbes et écoToxIcoLogiE - NAUTILE (France)
3 GRL - Groupe de Recherche de Lacq
4 CIRIMAT - Centre interuniversitaire de recherche et d'ingenierie des matériaux
5 Arkema - Arkema

The classical approach in ecotoxicological evaluation of chemical substances consists of conducting standardized bioassays on organism models. In this work, the potential impact of industrial multiwalled carbon nanotubes was investigated by ecotoxicological standardized procedures using aquatic organisms of different trophic levels, namely bacteria, green algae, invertebrates, fish, and amphibians. The results indicated (1) inhibition of growth in amphibians at 50 mg L¡1 and higher, and (2) no effects on daphnia and fish up to 100 mg L¡1. With the exception of algae (for which Fe deficiency is measured), it seems that the observed toxicity may be due to physiological effects in relation to the ingestion of carbon nanotubes not necessarily related to their intrinsic effects.

Mots clés: Multi-walled carbon nanotubes; Ecotoxicity; Standardized bioassays; Bacteria; Algae; Fish; Amphibians

Domaines: Matériaux; Ecotoxicologie; Matériaux

Florence Mouchet, Christian Gancet, Emmanuel Flahaut, Eric Pinelli, Jean–charles Boutonnet, et al.. International standardized procedures forin vivo evaluation of multi-walled carbon nanotube toxicity in water. Toxicological and Environmental Chemistry, 2016, vol. 98 (n° 8), pp. 829-847. ⟨10.1080/02772248.2015.1133818⟩. ⟨hal-01475094⟩