Pre-hatching fluoxetine-induced neurochemical, neurodevelopmental, and immunological changes in newly hatched cuttlefish

Flavie Bidel (1) , Carole Di Poi (1) , Boudjema Imarazene (2) , Noussithe Koueta (3) , Hélène Budzinski (3) , Pierre van Delft (3) , Cécile Bellanger (1) , Christelle Jozet-Alves (1)

1 GMPc - Groupe Mémoire et Plasticité comportementale
2 BOREA - Biologie des Organismes et Ecosystèmes Aquatiques
3 EPOC - Environnements et Paléoenvironnements OCéaniques

Embryonic and early postembryonic development of the cuttlefish Sepia officinalis (a cephalopod mollusk) occurs in coastal waters, an environment subject to considerable pressure from xenobiotic pollutants such as pharmaceutical residues. Given the role of serotonin in brain development and its interaction with neurodevelopmental functions, this study focused on fluoxetine (FLX), a selective serotonin reuptake inhibitor (SSRI, antidepressant). The goal was to determine the effects of subchronic waterborne FLX exposure (1 and 10 mu g L-1) during the last 15 days of embryonic development on neurochemical, neurodevelopmental, behavioral, and immunological endpoints at hatching. Our results showed for the first time that organic contaminants, such as FLX, could pass through the eggshell during embryonic development, leading to a substantial accumulation of this molecule in hatchlings. We also found that FLX embryonic exposure (1 and 10 mu g L-1) (1) modulated dopaminergic but not serotonergic neurotransmission, (2) decreased cell proliferation in key brain structures for cognitive and visual processing, (3) did not induce a conspicuous change in camouflage quality, and (4) decreased lysozyme activity. In the long term, these alterations observed during a critical period of development may impair complex behaviors of the juvenile cuttlefish and thus lead to a decrease in their survival. Finally, we suggest a different mode of action by FLX between vertebrate and non-vertebrate species and raise questions regarding the vulnerability of early life stages of cuttlefish to the pharmaceutical contamination found in coastal waters.

Mots clés: antidepressant treatment; Bioconcentration; body patterns; Brain maturation; camouflage; Cell proliferation; cell-proliferation; Cephalopods; fish betta-splendens; Immune system; medaka oryzias-latipes; Monoamines; personal care products; prefrontal cortex; Selective serotonin reuptake inhibitor (SSRI); sepia-officinalis; serotonin reuptake inhibitors; vertical lobe complex

Domaines: Neurosciences; Sciences du Vivant [q-bio]; Zoologie des invertébrés

Flavie Bidel, Carole Di Poi, Boudjema Imarazene, Noussithe Koueta, Hélène Budzinski, et al.. Pre-hatching fluoxetine-induced neurochemical, neurodevelopmental, and immunological changes in newly hatched cuttlefish. Environmental Science and Pollution Research, 2016, 23 (6), pp.5030--5045. ⟨10.1007/s11356-015-4591-7⟩. ⟨hal-01472649⟩