Insights into the Planktothrix genus: Genomic and metabolic comparison of benthic and planktic strains

Claire Pancrace (1, 2) , Marie-Anne Barny (1) , Reiko Ueoka (3) , Alexandra Calteau (4) , Thibault Scalvenzi (2) , Jacques Pédron (1) , Valérie Barbe (5) , Joern Piel (3) , Jean-François Humbert (1) , Muriel Gugger (2)

1 iEES - Institut d'écologie et des sciences de l'environnement de Paris
2 Collection des Cyanobactéries
3 ETH Zürich - Eidgenössische Technische Hochschule - Swiss Federal Institute of Technology [Zürich]
4 UMR 8030 - Génomique métabolique
5 GENOSCOPE - Genoscope - Centre national de séquençage [Evry]

Planktothrix is a dominant cyanobacterial genus forming toxic blooms in temperate freshwater ecosystems. We sequenced the genome of planktic and non planktic Planktothrix strains to better represent this genus diversity and life style at the genomic level. Benthic and biphasic strains are rooting the Planktothrix phylogenetic tree and widely expand the pangenome of this genus. We further investigated in silico the genetic potential dedicated to gas vesicles production, nitrogen fixation as well as natural product synthesis and conducted complementary experimental tests by cell culture, microscopy and mass spectrometry. Significant differences for the investigated features could be evidenced between strains of different life styles.

Mots clés: GAS VESICLE GENES; NONHETEROCYSTOUS CYANOBACTERIA; SECONDARY METABOLISM; EVOLUTIONARY ORIGIN; SEQUENCE ALIGNMENT; LAKE ZURICH; DIVERSITY

Mots clés: FILAMENTOUS CYANOBACTERIA; N-2 FIXATION; RUBESCENS

Domaines: Bactériologie; Génomique, Transcriptomique et Protéomique [q-bio.GN]

Claire Pancrace, Marie-Anne Barny, Reiko Ueoka, Alexandra Calteau, Thibault Scalvenzi, et al.. Insights into the Planktothrix genus: Genomic and metabolic comparison of benthic and planktic strains. Scientific Reports, 2017, 7, pp.41181. ⟨10.1038/srep41181⟩. ⟨hal-01470599⟩