Architecture and assembly of the Type VI secretion system.

Abdelrahim Zoued (1) , Yannick R Brunet , Eric Durand (1) , Marie-Stéphanie Aschtgen (1) , Laureen Logger (1) , Badreddine Douzi (2) , Laure Journet (1) , Christian Cambillau (2) , E. Cascales (1)

1 LISM - Laboratoire d'ingénierie des systèmes macromoléculaires
2 AFMB - Architecture et fonction des macromolécules biologiques

The Type VI secretion system (T6SS) delivers protein effectors to diverse cell types including prokaryotic and eukaryotic cells, therefore it participates in inter-bacterial competition and pathogenesis. The T6SS is constituted of an envelope-spanning complex anchoring a cytoplasmic tubular edifice. This tubular structure is evolutionarily, functionally and structurally related to the tail of contractile phages. It is composed of an inner tube tipped by a spike complex, and engulfed within a sheath-like structure. This structure assembles onto a platform called "baseplate" that is connected to the membrane sub-complex. The T6SS functions as a nano-crossbow: upon contraction of the sheath, the inner tube is propelled towards the target cell, allowing effector delivery. This review focuses on the architecture and biogenesis of this fascinating secretion machine, highlighting recent advances regarding the assembly of the membrane or tail complexes. This article is part of a Special Issue entitled: Protein trafficking and secretion in bacteria. Guest Editors: Anastassios Economou and Ross Dalbey.

Mots clés: Bacteria/chemistry/*pathogenicity; Bacterial Proteins/chemistry/*metabolism; Bacterial Secretion Systems/*genetics; Bacteriophages/chemistry/metabolism; Cytoplasm/metabolism; Eukaryotic Cells/metabolism; Prokaryotic Cells/metabolism; Assembly platform; Bacteriophage; Baseplate; Hcp; Inter-bacterial interactions; Secretion system; VgrG

Mots clés: Cell Membrane/chemistry/metabolism; Protein Transport/*genetics; Membrane complex; Microbial communities; Protein transport; Tail

Domaines: Biochimie, Biologie Moléculaire

Abdelrahim Zoued, Yannick R Brunet, Eric Durand, Marie-Stéphanie Aschtgen, Laureen Logger, et al.. Architecture and assembly of the Type VI secretion system.. Biochim. Biophys. Acta, 2014, 1843 (8), pp.1664--73. ⟨10.1016/j.bbamcr.2014.03.018⟩. ⟨hal-01458220⟩