Circadian RNA expression elicited by 3’-UTR IRAlu-paraspeckle associated elements

Abstract : Circadian clocks regulate rhythmic gene expression levels by means of mRNA oscillations that are mainly driven by post-transcriptional regulation. We identified a new post-transcriptional mechanism, which involves nuclear bodies called paraspeckles. Major components of paraspeckles including the long noncoding RNA Neat1, which is the structural component, and its major protein partners, as well as the number of paraspeckles, follow a circadian pattern in pituitary cells. Paraspeckles are known to retain within the nucleus RNAs containing inverted repeats of Alu sequences. We showed that a reporter gene in which these RNA duplex elements were inserted in the 3’-UTR region displayed a circadian expression. Moreover, circadian endogenous mRNA associated with paraspeckles lost their circadian pattern when paraspeckles were disrupted. This work not only highlights a new paraspeckle-based post-transcriptional mechanism involved in circadian gene expression but also provides the list of all mRNA associated with paraspeckles in the nucleus of pituitary cells
Type de document :
Article dans une revue
eLife, eLife Sciences Publication, 2016, 5, pp.e14837. 〈10.7554/eLife.14837〉
Liste complète des métadonnées

Littérature citée [39 références]  Voir  Masquer  Télécharger

https://hal.archives-ouvertes.fr/hal-01457643
Contributeur : Denis Becquet <>
Soumis le : mardi 7 mars 2017 - 14:04:58
Dernière modification le : lundi 9 avril 2018 - 18:31:34
Document(s) archivé(s) le : jeudi 8 juin 2017 - 13:52:07

Fichier

e14837-download.pdf
Fichiers éditeurs autorisés sur une archive ouverte

Identifiants

Collections

Citation

Manon Torres, Denis Becquet, Marie-Pierre Blanchard, Séverine Guillen, Bénédicte Boyer, et al.. Circadian RNA expression elicited by 3’-UTR IRAlu-paraspeckle associated elements. eLife, eLife Sciences Publication, 2016, 5, pp.e14837. 〈10.7554/eLife.14837〉. 〈hal-01457643〉

Partager

Métriques

Consultations de la notice

120

Téléchargements de fichiers

36