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Circadian RNA expression elicited by 3’-UTR IRAlu-paraspeckle associated elements
Abstract : Circadian clocks regulate rhythmic gene expression levels by means of mRNA oscillations that are
mainly driven by post-transcriptional regulation. We identified a new post-transcriptional
mechanism, which involves nuclear bodies called paraspeckles. Major components of paraspeckles
including the long noncoding RNA Neat1, which is the structural component, and its major protein
partners, as well as the number of paraspeckles, follow a circadian pattern in pituitary cells.
Paraspeckles are known to retain within the nucleus RNAs containing inverted repeats of Alu
sequences. We showed that a reporter gene in which these RNA duplex elements were inserted in
the 3’-UTR region displayed a circadian expression. Moreover, circadian endogenous mRNA
associated with paraspeckles lost their circadian pattern when paraspeckles were disrupted. This
work not only highlights a new paraspeckle-based post-transcriptional mechanism involved in
circadian gene expression but also provides the list of all mRNA associated with paraspeckles in the
nucleus of pituitary cells
Cited literature [39 references]
https://hal.archives-ouvertes.fr/hal-01457643
Contributor : Denis Becquet Connect in order to contact the contributor
Submitted on : Tuesday, March 7, 2017 - 2:04:58 PM
Last modification on : Wednesday, November 3, 2021 - 4:04:23 AM
Long-term archiving on: : Thursday, June 8, 2017 - 1:52:07 PM
Contributor : Denis Becquet Connect in order to contact the contributor
Submitted on : Tuesday, March 7, 2017 - 2:04:58 PM
Last modification on : Wednesday, November 3, 2021 - 4:04:23 AM
Long-term archiving on: : Thursday, June 8, 2017 - 1:52:07 PM
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