In vitro impact of a whey protein isolate (WPI) and collagen hydrolysates (CHs) on B16F10 melanoma cells proliferation

G.A. Castro (1) , D.A. Maria (2) , Said Bouhallab (3) , V.C. Sgarbieri (1)

1 Food and Nutrition Department, Food Engineering Faculty
2 Biochemistry and Biophysics Laboratory
3 STLO - Science et Technologie du Lait et de l'Oeuf

Porcine skin gelatine presented anti-tumoral effect on murine hepatoma cells (MH134), inducing programmed cell death (apoptosis). Whey proteins (mainly lactoferrin) have been investigated for cancer prevention and treatment. Objective: Investigation of the inhibitory capacity on melanoma cells (B16F10) proliferation and the influence on % distribution of cell cycle phases, in the presence of various concentrations of whey protein isolate (WPI), bovine collagen hydrolysate (BCH) or its fractions. Methods: The permeate fraction BCH-P1 (molecular mass, MM 2.5 kDa) was further fractionated into five retentate fractions (R1–R5) by ultrafiltration membranes and into four fractions (F1–F4) by reverse phase chromatography. The permeate BCH-P1 and all its fractions were comparatively tested against a negative control (B16F10 cells + culture medium), and also against a positive control (B16F10 + culture medium + WPI). Results: The inhibitory concentrations for 50% of B16F10 cells (IC50) ranged from 0.19 to 156.9 mg/mL for all these proteins evaluated. The most inhibitory fractions of the BCH hydrolysate were BCH-P1 and F1– F4 with IC50 concentrations below 1 mg/mL. Changes in cell cycle phases were characterized by a general decrease in the G2/M phase that emphasizes growth arrest, some increase in phase S (BCH-P1 and F4) but a strong increase in G0/G1 phase for BCH-P1 and F4. Caspase-3 expression increased significantly in all media containing F and R fractions, and also in the presence of BCH or WPI. Apoptosis was extremely high at low concentration (400 mg/mL) of the F1–F3 fractions. Conclusion: It is suggested that a mechanism for tumorigenesis inhibition may involve the caspases cascade and apoptosis.

Mots clés: WHEY PROTEIN; APOPTOSIS; COLLAGEN HYDROLYSATES; CELL CYCLE PHASES; B16F10 MELANOM; milk protein; protein

Mots clés: protéine du lait; protéine; apoptose

Domaines: Sciences du Vivant [q-bio]

G.A. Castro, D.A. Maria, Said Bouhallab, V.C. Sgarbieri. In vitro impact of a whey protein isolate (WPI) and collagen hydrolysates (CHs) on B16F10 melanoma cells proliferation. Journal of Dermatological Science, 2009, 56, pp.51-57. ⟨10.1016/j.jdermsci.2009.06.016⟩. ⟨hal-01454037⟩