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Article Dans Une Revue Biophysical Journal Année : 2016

Dynamic Optical Displacement Spectroscopy to Quantify Biomembrane Bending Fluctuations

Cornelia Monzel
  • Fonction : Auteur
Ana-Suncana Smith
  • Fonction : Auteur
Rudolf Merkel
  • Fonction : Auteur

Résumé

Stochastic bending fluctuations of biomembranes are of high physiological importance, e.g., for preventing adhesion between red blood cells or for the establishment of cell substrate contacts in cell adhesion. Unfortunately, especially in nucleated cells they are notoriously difficult to quantify. To meet this challenge we developed a new technique based on Fluorescence Correlation Spectroscopy (FCS). We use comparatively high labeling of the biomembrane (∼10000 fluorophores in the focal spot) to suppress all contributions of lateral diffusion to fluctuations of the fluorescence intensity. Under these conditions all intensity variations arise from axial motion of the membrane. Best results are achieved when the focus is placed slightly above or below the membrane. The resolution, as estimated for ideal membranes is 20 nm spatial and 10 µs temporal.

Domaines

Biophysique

Dates et versions

hal-01452672 , version 1 (02-02-2017)

Identifiants

Citer

Cornelia Monzel, Daniel Schmidt, Udo Seifert, Ana-Suncana Smith, Kheya Sengupta, et al.. Dynamic Optical Displacement Spectroscopy to Quantify Biomembrane Bending Fluctuations. Biophysical Journal, 2016, 110 (3, suppl. 1), pp.487A. ⟨10.1016/j.bpj.2015.11.2603⟩. ⟨hal-01452672⟩
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