Desulfotomaculum aquiferis sp nov and Desulfotomaculum profundi sp nov., isolated from a deep natural gas storage aquifer

Abstract : Two novel strictly anaerobic bacteria, strains Bs105(T) and Bs107(T), were isolated from a deep aquifer-derived hydrocarbonoclastic community. The cells were rod-shaped, not motile and had terminal spores. Phylogenetic affiliation and physiological properties revealed that these isolates belong to two novel species of the genus Desulfotomaculum. Optimal growth temperatures for strains Bs105(T) and Bs107(T) were 42 and 45 degrees C, respectively. The estimated G+C content of the genomic DNA was 42.9 and 48.7 mol%. For both strains, the major cellular fatty acid was palmitate (C-16 : 0). Specific carbon fatty acid signatures of Gram-positive bacteria (iso-C-17 : 0) and sulfate-reducing bacteria (C(17 : 0)cyc) were also detected. An insertion was revealed in one of the two 16S rRNA gene copies harboured by strain Bs107(T). Similar insertions have previously been highlighted among moderately thermophilic species of the genus Desulfotomaculum. Both strains shared the ability to oxidize aromatic acids (Bs105(T) : hydroquinone, acetophenone, paratoluic acid, 2-phenylethanol, trans-cinnamic acid, 4-hydroxybenzaldehyde, benzyl alcohol, benzoic acid 4-hydroxybutyl ester; Bs107(T) : ortho-toluic acid, benzoic acid 4-hydroxybutyl ester). The names Desulfotomaculum aquiferis sp. nov. and Desulfotomaculum profundi sp. nov. are proposed for the type strains Bs105(T) (= DSM 24088(T) = JCM 31386(T)) and Bs107(T) (= DSM 24093(T) = JCM 31387(T)).
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International Journal of Systematic and Evolutionary Microbiology, Microbiology Society, 2016, 66 (11), pp.4329-4338. 〈10.1099/ijsem.0.001352〉
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https://hal.archives-ouvertes.fr/hal-01443240
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Soumis le : dimanche 22 janvier 2017 - 21:17:00
Dernière modification le : vendredi 29 juin 2018 - 10:56:08

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Sabrina Berlendis, Magali Ranchou-Peyruse, Marie-Laure Fardeau, Jean-Francois Lascourreges, Manon Joseph, et al.. Desulfotomaculum aquiferis sp nov and Desulfotomaculum profundi sp nov., isolated from a deep natural gas storage aquifer. International Journal of Systematic and Evolutionary Microbiology, Microbiology Society, 2016, 66 (11), pp.4329-4338. 〈10.1099/ijsem.0.001352〉. 〈hal-01443240〉

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