Cutting Edge: Eomesodermin Is Sufficient To Direct Type 1 Innate Lymphocyte Development into the Conventional NK Lineage

Olga Pikovskaya , Julie Chaix (1) , Nyanza J. Rothman , Amelie Collins , Yen-Hua Chen , Anna M. Scipioni , Eric Vivier (1) , Steven L. Reiner

1 CIML - Centre d'Immunologie de Marseille - Luminy

Type 1 innate lymphocytes comprise two developmentally divergent lineages, type 1 helper innate lymphoid cells (hILC1s) and conventional NK cells (cNKs). All type 1 innate lymphocytes (ILCs) express the transcription factor T-bet, but cNKs additionally express Eomesodermin (Eomes). We show that deletion of Eomes alleles at the onset of type 1 ILC maturation using NKp46-Cre imposes a substantial block in cNK development. Formation of the entire lymphoid and nonlymphoid type 1 ILC compartment appears to require the semiredundant action of both T-bet and Eomes. To determine if Eomes is sufficient to redirect hILC1 development to a cNK fate, we generated transgenic mice that express Eomes when and where T-bet is expressed using Tbx21 locus control to drive expression of Eomes codons. Ectopic Eomes induces cNK-like properties across the lymphoid and nonlymphoid type 1 ILC compartments. Subsequent to their divergent lineage specification, hILC1s and cNKs thus possess substantial developmental plasticity.

Mots clés: Tbx2; Tbx1 protein; Mice; Lymphocytes

Domaines: Immunologie

Olga Pikovskaya, Julie Chaix, Nyanza J. Rothman, Amelie Collins, Yen-Hua Chen, et al.. Cutting Edge: Eomesodermin Is Sufficient To Direct Type 1 Innate Lymphocyte Development into the Conventional NK Lineage. Journal of Immunology, 2016, 196 (4), pp.1449-1454. ⟨10.4049/jimmunol.1502396⟩. ⟨hal-01438533⟩