Prokaryotic diversity in a Tunisian hypersaline lake, Chott El Jerid

Abstract : Prokaryotic diversity was investigated in a Tunisian salt lake, Chott El Jerid, by quantitative real-time PCR, denaturing gradient gel electrophoresis (DGGE) fingerprinting methods targeting the 16S rRNA gene and culture-dependent methods. Two different samples S1-10 and S2-10 were taken from under the salt crust of Chott El Jerid in the dry season. DGGE analysis revealed that bacterial sequences were related to Firmicutes, Proteobacteria, unclassified bacteria, and Deinococcus-Thermus phyla. Anaerobic fermentative and sulfate-reducing bacteria were also detected in this ecosystem. Within the domain archaea, all sequences were affiliated to Euryarchaeota phylum. Quantitative real-time PCR showed that 16S rRNA gene copy numbers of bacteria was 5 x 10(6) DNA copies g(-1) whereas archaea varied between 5 x 10(5) and 10(6) DNA copies g(-1) in these samples. Eight anaerobic halophilic fermentative bacterial strains were isolated and affiliated with the species Halanaerobium alcaliphilum, Halanaerobium saccharolyticum, and Sporohalobacter salinus. These data showed an abundant and diverse microbial community detected in the hypersaline thalassohaline environment of Chott El Jerid.
Type de document :
Article dans une revue
Extremophiles, Springer Verlag, 2016, 20 (2), pp.125-138. 〈10.1007/s00792-015-0805-7〉
Liste complète des métadonnées

https://hal.archives-ouvertes.fr/hal-01437952
Contributeur : Marianne Quéméneur <>
Soumis le : mardi 17 janvier 2017 - 14:31:31
Dernière modification le : vendredi 22 juin 2018 - 18:28:03

Identifiants

Citation

Manel Ben Abdallah, Fatma Karray, Najla Mhiri, Nan Mei, Marianne Quemeneur, et al.. Prokaryotic diversity in a Tunisian hypersaline lake, Chott El Jerid. Extremophiles, Springer Verlag, 2016, 20 (2), pp.125-138. 〈10.1007/s00792-015-0805-7〉. 〈hal-01437952〉

Partager

Métriques

Consultations de la notice

258