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The harmonized INFOGEST in vitro digestion method: From knowledge to action

Lotti Egger 1 Olivia Ménard 2 Cristina Delgado-Andrade 3 Paula Alvito 4 Ricardo Assunção 5 Simon Balance 6 Reyes Barberá 7 Andre Brodkorb 8 Thomas Cattenoz 9 Alfonso Clemente 3 Irene Comi 10 Didier Dupont 2 Guadalupe Garcia-Llatas 7 María Jesús Lagarda 7 Steven Le Feunteun 9 Lonneke Janssenduijghuijsen 11 Sibel Karakaya 12 Uri Lesmes 13 Alan R. Mackie 14 Carla Martins 15 Anne Meynier 16 Beatriz Miralles 17 Brent S. Murray 18 Anne Pihlanto 19 Gianluca Picariello 20 Claudia N. Santos 21, 22 Sebnem Simsek 12 Isidra Recio 17 Neil Rigby 23 Laurie-Eve Rioux 24 Helena Stoffers 25 Ana Tavares 15 Lucelia Tavares 26, 22 Sylvie Turgeon 24 Ellen K. Ulleberg 10 Gerd E. Vegarud 27 Guy Vergères 1 Reto Portmann 1
Abstract : Within the active field of in vitro digestion in food research, the COST Action INFOGEST aimed to harmonize in vitro protocols simulating human digestion on the basis of physiologically inferred conditions. A harmonized static in vitro digestion (IVD) method was recently published as a primary output from this network. To validate this protocol, inter-laboratory trials were conducted within the INFOGEST network. A first study was performed using skim milk powder (SMP) as a model food and served to compare the different in-house digestion protocols used among the INFOGEST members. In a second inter-laboratory study applying the harmonized protocol, thedegree of consistency in protein hydrolysis was investigated. Analysis of the hydrolyzed proteins, after the gastric and intestinal phases, showed that caseins were mainly hydrolyzed during the gastric phase, whereas β- lactoglobulin was, as previously shown, resistant to pepsin. Moreover, generation of free amino acids occurred mainly during the intestinal phase. The study also showed that a few critical steps were responsible for the remaining inter-laboratory variability. The largest deviations arose from the determination of pepsin activity. Therefore, this step was further clarified, harmonized, and implemented in a third inter-laboratory study. The presentwork gives an overviewof all three inter-laboratory studies, showing that the IVD INFOGEST method has led to an increased consistency that enables a better comparability of in vitro digestion studies in the future.
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Submitted on : Friday, January 13, 2017 - 8:29:27 PM
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Lotti Egger, Olivia Ménard, Cristina Delgado-Andrade, Paula Alvito, Ricardo Assunção, et al.. The harmonized INFOGEST in vitro digestion method: From knowledge to action. Food Research International, 2016, 88, pp.217-225. ⟨10.1016/j.foodres.2015.12.006⟩. ⟨hal-01435380⟩



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