High protein flexibility and reduced hydration water dynamics are key pressure adaptive strategies in prokaryotes

Abstract : Water and protein dynamics on a nanometer scale were measured by quasi-elastic neutron scattering in the piezophile archaeon Thermococcus barophilus and the closely related pressure-sensitive Thermococcus kodakarensis, at 0.1 and 40 MPa. We show that cells of the pressure sensitive organism exhibit higher intrinsic stability. Both the hydration water dynamics and the fast protein and lipid dynamics are reduced under pressure. In contrast, the proteome of T. barophilus is more pressure sensitive than that of T. kodakarensis. The diffusion coefficient of hydration water is reduced, while the fast protein and lipid dynamics are slightly enhanced with increasing pressure. These findings show that the coupling between hydration water and cellular constituents might not be simply a master-slave relationship. We propose that the high flexibility of the T. barophilus proteome associated with reduced hydration water may be the keys to the molecular adaptation of the cells to high hydrostatic pressure.
Type de document :
Article dans une revue
Scientific Reports, Nature Publishing Group, 2016, 6, <10.1038/srep32816>
Liste complète des métadonnées


https://hal.archives-ouvertes.fr/hal-01427793
Contributeur : Frank Thomas <>
Soumis le : lundi 29 mai 2017 - 15:54:59
Dernière modification le : vendredi 2 juin 2017 - 01:05:06

Fichier

srep32816.pdf
Publication financée par une institution

Identifiants

Collections

Citation

N. Martinez, G. Michoud, A. Cario, J. Ollivier, Bruno Franzetti, et al.. High protein flexibility and reduced hydration water dynamics are key pressure adaptive strategies in prokaryotes. Scientific Reports, Nature Publishing Group, 2016, 6, <10.1038/srep32816>. <hal-01427793>

Partager

Métriques

Consultations de
la notice

93

Téléchargements du document

21