Is There a Trojan-Horse Effect during Magnetic Nanoparticles and Metalloid Cocontamination of Human Dermal Fibroblasts? - Archive ouverte HAL Accéder directement au contenu
Article Dans Une Revue Environmental Science and Technology Année : 2012

Is There a Trojan-Horse Effect during Magnetic Nanoparticles and Metalloid Cocontamination of Human Dermal Fibroblasts?

Olivier Proux
Fabio Ziarelli
  • Fonction : Auteur

Résumé

This study investigates the issue of nanoparticles/pollutants cocontamination. By combining viability assays, physicochemical and structural analysis (to probe the As speciation and valence), we assessed how gamma Fe2O3 nanoparticles can affect the cytotoxicity, the intra- and extracellular speciation of As(III). Human dermal fibroblasts were contaminated with gamma Fe2O3 nanoparticles and As(III) considering two scenarios: (i) a simultaneous coinjection of the nanoparticles and As, and (ii) an injection of the I: nanoparticles after 24 h of As adsorption in water. In both scenarios, we did not notice significant changes on the nanoparticles surface charge (zeta potential similar to -10 mV) nor hydrodynamic diameters (similar to 9.50 nm) after 24 h. We demonstrated that the coinjection of gamma Fe2O3 nanoparticles and As in the cellular media strongly affects the complexation of the intracellular As with thiol groups. This significantly increases at low doses the cytotoxicity of the As nonadsorbed at the surface of the nanoparticles. However, once As is adsorbed at the surface the desorption is very weak in the culture medium. This fraction of As strongly adsorbed at the surface is significantly less cytotoxic than As itself. On the basis of our data and the thermodynamics, we demonstrated that any disturbance of the biotransformation mechanisms by the nanoparticles (i.e., surface complexation of thiol groups with the iron atoms) is likely to be responsible for the increase of the As adverse effects at low doses.
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Dates et versions

hal-01426196 , version 1 (04-01-2017)

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Melanie Auffan, Jérôme Rose, Olivier Proux, Armand Masion, Wei Liu, et al.. Is There a Trojan-Horse Effect during Magnetic Nanoparticles and Metalloid Cocontamination of Human Dermal Fibroblasts?. Environmental Science and Technology, 2012, 46 (19), pp.10789-10796. ⟨10.1021/es302493s⟩. ⟨hal-01426196⟩
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