Estrogen receptor preparation effects on the receptor–DNA interaction by surface plasmon resonance

Sandrine Bayle (1) , Joel Chopineau (2, 3) , Benoit Roig (4, 3) , Denis Habauzit (1, 5)

1 LGEI - Laboratoire de Génie de l'Environnement Industriel
2 ICGM - Institut Charles Gerhardt Montpellier - Institut de Chimie Moléculaire et des Matériaux de Montpellier
3 UNIMES - Université de Nîmes
4 CHROME - Détection, évaluation, gestion des risques CHROniques et éMErgents (CHROME) / Université de Nîmes
5 Irset - Institut de recherche en santé, environnement et travail

Up to now, several studies have investigated estrogen receptor (ER)–estrogen response element (ERE) interaction using biosensors such as surface plasmon resonance. These strategies have aimed to understand the molecular mechanism of such interaction as well as the effect of the ligand on this interaction. These approaches start to be used to determine the mechanisms of protein/DNA interaction, in particular in the context of drug discovery or environmental applications. However, some physical and biochemical parameters (incubation time, temperature, protease inhibitor cocktail, and bovine serum albumin (BSA)) are not completely described in the literature and could deeply modify the obtained results. This paper aims to focus not only on the preliminary steps of sample preparation such as protein thawing and incubation conditions (time and temperature) but also on the evaluation of protease inhibitor cocktail and BSA effect on the measurement of ER–ERE interactions.

Mots clés: Dimerization; Plasmons; Proteins; Resonance; Temperature; Biochemical parameters; Bovine serum albumins; Estrogen response elements; Molecular mechanism; Sample preparation; Surface plasmon resonance; Estrogen receptor; Environmental applications; Estrogen; Body fluids

Domaines: Biochimie, Biologie Moléculaire

Sandrine Bayle, Joel Chopineau, Benoit Roig, Denis Habauzit. Estrogen receptor preparation effects on the receptor–DNA interaction by surface plasmon resonance. Analytical and Bioanalytical Chemistry, 2016, 408 (29), pp.8257-8262. ⟨10.1007/s00216-016-9967-0⟩. ⟨hal-01418341⟩