Accumulation and Secretion of Coumarinolignans and other Coumarins in Arabidopsis thaliana Roots in Response to Iron Deficiency at High pH

Patricia Siso-Terraza (1) , Adrian Luis-Villarroya (1) , Pierre Fourcroy (2) , Jean-Francois Briat (2) , Anunciacion Abadia (1) , Frédéric Gaymard (2) , Javier Abadia (1) , Ana Alvarez-Fernandez (1)

1 Plant Nutrition, Aula Dei Experimental Station
2 BPMP - Biochimie et Physiologie Moléculaire des Plantes

Root secretion of coumarin-phenolic type compounds has been recently shown to be related to Arabidopsis thaliana tolerance to Fe deficiency at high pH. These studies revealed the identity of a few simple coumarins occurring in roots and exudates of Fe-deficient A. thaliana plants, and left open the possible existence of other unknown phenolics. We used HPLC-UV-VIS-ESI-MS(TOF),HPLC/ESI-MSn(ion trap) and HPLC-ESI-MS-MS(Q-TOF) to characterize (identify and quantify) phenolic-type compounds accumulated in roots or secreted into the nutrient solution of A. thaliana plants in response to Fe deficiency. Plants grown with or without Fe and using nutrient solutions buffered at pH 5.5 or 7.5 enabled to identify an array of phenolics. These include several coumarinolignans not previously reported in A. thaliana (cleomiscosins A, B, C and D and the 5’-hydroxycleomiscosins A and/or B), as well as some coumarin precursors (ferulic acid and coniferyl and sinapyl aldehydes), and previously reported cathecol (fraxetin) and non-cathecol coumarins (scopoletin, isofraxidin and fraxinol), some of them in hexoside forms not previously characterized. The production and secretion of phenolics were more intense when the plant accessibility to Fe was diminished and the plant Fe status deteriorated, as it occurs when plants are grown in the absence of Fe at pH 7.5. Aglycones and hexosides of the four coumarins were abundant in roots, whereas only the aglycone forms could be quantified in the nutrient solution. A comprehensive quantification of coumarins, first carried out in this study, revealed that the catechol coumarin fraxetin was predominant in exudates (but not in roots) of Fe-deficient A. thaliana plants grown at pH 7.5. Also, fraxetin was able to mobilize efficiently Fe from a Fe(III)-oxide at pH 5.5 and pH 7.5. On the other hand, non-catechol coumarins were much less efficient in mobilizing Fe and were present in much lower concentrations, making unlikely that they could play a role in Fe mobilization. The structural features of the array of coumarin type-compounds produced suggest some can mobilize Fe from the soil and others can be more efficient as allelochemicals.

Mots clés: Coumarin; Cleomiscosin; coumarin; root; Fraxetin; iron nutrition; Mass Spectrometry; Root secretion

Mots clés: racine; arabidopsis; coumarine; absorption du fer; oxydation abiotique; carence en fer

Domaines: Biologie végétale

Patricia Siso-Terraza, Adrian Luis-Villarroya, Pierre Fourcroy, Jean-Francois Briat, Anunciacion Abadia, et al.. Accumulation and Secretion of Coumarinolignans and other Coumarins in Arabidopsis thaliana Roots in Response to Iron Deficiency at High pH. Frontiers in Plant Science, 2016, 7, pp.1711. ⟨10.3389/fpls.2016.01711⟩. ⟨hal-01417731⟩