Transition from physical activity to inactivity increases skeletal muscle miR-148b content and triggers insulin resistance

Caroline Gastebois (1) , Stéphanie Chanon (1) , Sophie Rome (1) , Christine Durand (1) , Elise Pelascini (2) , Audrey Jalabert (1) , Vanessa Euthine (1) , Vincent Pialoux (3) , Stéphane Blanc (4) , Chantal Simon (1) , Etienne Lefai (1)

1 CarMeN - Cardiovasculaire, métabolisme, diabétologie et nutrition
2 Département de Chirurgie Digestive et Bariatrique Hospices Civils de Lyon
3 LIBM - Laboratoire Interuniversitaire de Biologie de la Motricité
4 DEPE-IPHC - Département Ecologie, Physiologie et Ethologie

This study investigated miR-148b as a potential physiological actor of physical inactivity-induced effects in skeletal muscle. By using animal and human protocols , we demonstrated that the early phase of transition toward inactivity was associated with an increase in muscle miR-148b content, which triggered the downregulation of NRAS and ROCK1 target genes. Using human myo-tubes, we demonstrated that overexpression of miR-148b decreased NRAS and ROCK1 protein levels, and PKB phosphorylation and glucose uptake in response to insulin. Increase in muscle miR-148b content might thus participate in the decrease in insulin sensitivity at the whole body level during the transition toward physical inactivity.

Mots clés: miRNA; physical inactivity; Insulin-signaling pathway; muscle tissue; myotube

Domaines: Physiologie [q-bio.TO]

Caroline Gastebois, Stéphanie Chanon, Sophie Rome, Christine Durand, Elise Pelascini, et al.. Transition from physical activity to inactivity increases skeletal muscle miR-148b content and triggers insulin resistance. Physiological Reports, 2016, 4 (17), pp.e12902. ⟨10.14814/phy2.12902⟩. ⟨hal-01398592⟩