Skip to Main content Skip to Navigation
Journal articles

A fast and robust UHPLC-MRM-MS method to characterize and quantify grape skin tannins after chemical depolymerization

Abstract : A rapid, sensitive, and selective analysis method using ultra high performance liquid chromatography coupled with triple-quadrupole mass spectrometry (UHPLC-QqQ-MS) has been developed for the characterization and quantification of grape skin flavan-3-ols after acid-catalysed depolymerization in the presence of phloroglucinol (phloroglucinolysis). The compound detection being based on specific MS transitions in Multiple Reaction Monitoring (MRM) mode, this fast gradient robust method allows analysis of constitutive units of grape skin proanthocyanidins, including some present in trace amounts, in a single injection, with a throughput of 6 samples per hour. This method was applied to a set of 214 grape skin samples from 107 different red and white grape cultivars grown under two conditions in the vineyard, irrigated or non-irrigated. The results of triplicate analyses confirmed the robustness of the method, which was thus proven to be suitable for high-throughput and large-scale metabolomics studies. Moreover, these preliminary results suggest that analysis of tannin composition is relevant to investigate the genetic bases of grape response to drought.
Document type :
Journal articles
Complete list of metadata

Cited literature [17 references]  Display  Hide  Download

https://hal.archives-ouvertes.fr/hal-01397454
Contributor : Archive Ouverte Prodinra <>
Submitted on : Tuesday, November 15, 2016 - 8:22:49 PM
Last modification on : Tuesday, May 18, 2021 - 8:06:02 AM

File

molecules-21-01409.pdf
Publication funded by an institution

Identifiers

Citation

Lucie Pinasseau, Arnaud Verbaere, Maryline Roques, Emmanuelle Meudec, Anna Vallverdu Queralt, et al.. A fast and robust UHPLC-MRM-MS method to characterize and quantify grape skin tannins after chemical depolymerization. Molecules, MDPI, 2016, 21 (10), 13 p. ⟨10.3390/molecules21101409⟩. ⟨hal-01397454⟩

Share

Metrics

Record views

1054

Files downloads

625