How to correct inner filter effects altering 3D fluorescence spectra by using a mirrored cell
Résumé
In this paper we present a new correction method of inner filter effects that occurs when measuring fluorescence Excitation-Emission Matrices (EEM) of concentrated solutions. While traditional method requires absorption measurement or sample dilution(s), the Mirrored Cell Approach (MCA) only requires two different EEM of the considered sample: a first one using a traditional cell and a second one using a mirrored cell. The mathematical relationship between both models is originally exploited to obtain a simple numerical correction. Method is validated using a set of known mixtures. In addition we show that advanced multilinear analysis can be efficiently applied on to the corrected EEM. ?? 2013 Elsevier B.V.