Skip to Main content Skip to Navigation
Journal articles

How to correct inner filter effects altering 3D fluorescence spectra by using a mirrored cell

Abstract : In this paper we present a new correction method of inner filter effects that occurs when measuring fluorescence Excitation-Emission Matrices (EEM) of concentrated solutions. While traditional method requires absorption measurement or sample dilution(s), the Mirrored Cell Approach (MCA) only requires two different EEM of the considered sample: a first one using a traditional cell and a second one using a mirrored cell. The mathematical relationship between both models is originally exploited to obtain a simple numerical correction. Method is validated using a set of known mixtures. In addition we show that advanced multilinear analysis can be efficiently applied on to the corrected EEM. ?? 2013 Elsevier B.V.
Document type :
Journal articles
Complete list of metadata

https://hal.archives-ouvertes.fr/hal-01394612
Contributor : Stéphane Mounier <>
Submitted on : Wednesday, November 9, 2016 - 2:44:02 PM
Last modification on : Wednesday, October 14, 2020 - 4:21:31 AM

Identifiers

Collections

Citation

Xavier Luciani, Roland Redon, Stéphane Mounier. How to correct inner filter effects altering 3D fluorescence spectra by using a mirrored cell. Chemometrics and Intelligent Laboratory Systems, 2013, 126, pp.91--99. ⟨10.1016/j.chemolab.2013.04.014⟩. ⟨hal-01394612⟩

Share

Metrics

Record views

159