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Article Dans Une Revue Chemometrics and Intelligent Laboratory Systems Année : 2013

How to correct inner filter effects altering 3D fluorescence spectra by using a mirrored cell

Résumé

In this paper we present a new correction method of inner filter effects that occurs when measuring fluorescence Excitation-Emission Matrices (EEM) of concentrated solutions. While traditional method requires absorption measurement or sample dilution(s), the Mirrored Cell Approach (MCA) only requires two different EEM of the considered sample: a first one using a traditional cell and a second one using a mirrored cell. The mathematical relationship between both models is originally exploited to obtain a simple numerical correction. Method is validated using a set of known mixtures. In addition we show that advanced multilinear analysis can be efficiently applied on to the corrected EEM. ?? 2013 Elsevier B.V.

Dates et versions

hal-01394612 , version 1 (09-11-2016)

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Xavier Luciani, Roland Redon, Stéphane Mounier. How to correct inner filter effects altering 3D fluorescence spectra by using a mirrored cell. Chemometrics and Intelligent Laboratory Systems, 2013, 126, pp.91--99. ⟨10.1016/j.chemolab.2013.04.014⟩. ⟨hal-01394612⟩

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