Functional and structural responses of soil N-cycling microbial communities to the herbicide mesotrione: a dose-effect microcosm approach

Abstract : Microbial communities driving the nitrogen cycle contribute to ecosystem services such as crop production and air, soil, and water quality. The responses to herbicide stress of ammonia-oxidizing and ammonia-denitrifying microbial communities were investigated by an analysis of changes in structure-function relationships. Their potential activities, abundances (quantitative PCR), and genetic structure (denaturing gradient gel electrophoresis) were assessed in a microcosm experiment. The application rate (1 × FR, 0.45 μg g(-1) soil) of the mesotrione herbicide did not strongly affect soil N-nutrient dynamics or microbial community structure and abundances. Doses of the commercial product Callisto® (10 × FR and 100 × FR) or pure mesotrione (100 × FR) exceeding field rates induced short-term inhibition of nitrification and a lasting stimulation of denitrification. These effects could play a part in the increase in soil ammonium content and decrease in nitrate contents observed in treated soils. These functional impacts were mainly correlated with abundance shifts of ammonia-oxidizing Bacteria (AOB) and Archaea (AOA) or denitrifying bacteria. The sustained restoration of nitrification activity, from day 42 in the 100 × FR-treated soils, was likely promoted by changes in the community size and composition of AOB, which suggests a leading role, rather than AOA, for soil nitrification restoration after herbicide stress. This ecotoxicological community approach provides a nonesuch multiparameter assessment of responses of N-cycling microbial guilds to pesticide stress.
Document type :
Journal articles
Complete list of metadatas

https://hal.archives-ouvertes.fr/hal-01394067
Contributor : Genevieve Bricheux <>
Submitted on : Tuesday, November 8, 2016 - 3:39:11 PM
Last modification on : Thursday, March 21, 2019 - 2:32:06 PM

Identifiers

Citation

Olivier Crouzet, Franck Poly, Frédérique Bonnemoy, David Bru, Isabelle Batisson, et al.. Functional and structural responses of soil N-cycling microbial communities to the herbicide mesotrione: a dose-effect microcosm approach. Environmental Science and Pollution Research, Springer Verlag, 2016, 23 (5), pp.4207 - 4217. ⟨10.1007/s11356-015-4797-8⟩. ⟨hal-01394067⟩

Share

Metrics

Record views

218