Microbial treatment of water containing the bacteria Enterococcus faecalis and Escherichia coli in microcosm using Artemesia annua plant extract: Hierarchical order of some abiotic factors affecting cell’s cultivability

O. B. Mobili (1) , C. Lontsi Djimeli (1) , L. M. Moungang (1) , O Allaadhin (2) , A Tamsa Arfao (1) , Joseph Mabingui (2) , Geneviève Bricheux (3) , Moïse M Nola (1) , Télesphore Sime-Ngando (3)

1 FS - Faculté des Sciences [Yaoundé I]
2 Faculté des Sciences, Laboratoire d'Hydrosciences Lavoisier (LHL)
3 LMGE - Laboratoire Microorganismes : Génome et Environnement

Phytotherapy is increasingly used against infections in many parts of the world. Plants of the genus Artemisia, Asteraceae, are among those most exploited and several of its species were thus described as having antibacterial and antifungal effects. The infusions of Artemesia annua has been indicated as having disinfecting capacity in bacteriological contaminated water. The cultivability of Enterococcus faecalis and Escherichia coli was carried out in different extract solutions of A. annua aimed at describing the order of importance of some abiotic factors on this process. The bacterial abundance undergoes temporal variation with respect to pH of extract, A. annua extract and glucose concentrations, light intensity. The hierarchical organization of these factors has been carried out by expressing percentage and ranking in descending order of the sum of squares of each MANOVA test factor. It showed that cultivability of E. faecalis in A. annua extract solutions was influenced by the incubation durations, followed by the type of dilutions, light intensity, pH of extract, concentration of A. annua extract, and glucose concentrations. On the contrary, the cultivability of E. coli in A. annua extract solutions was influenced by the incubation durations, pH of infusion, type of dilutions, concentrations of A. annua extract, glucose concentrations and light intensity. The increase of the incubation durations, light intensity and pH of extract solutions caused a significant (P≤0.01) decrease of the effect of A. annua on E. faecalis and E. coli cultivability.

Mots clés: microbial treatment; cell's cultivability; impacting factors; A annua plant extract; water

Domaines: Biotechnologies; Bactériologie; Ingénierie de l'environnement

O. B. Mobili, C. Lontsi Djimeli, L. M. Moungang, O Allaadhin, A Tamsa Arfao, et al.. Microbial treatment of water containing the bacteria Enterococcus faecalis and Escherichia coli in microcosm using Artemesia annua plant extract: Hierarchical order of some abiotic factors affecting cell’s cultivability. European Journal of Biotechnology and Bioscience, 2016, 4 (9), pp.16-26. ⟨hal-01392643⟩