Membrane-associated GRP78 helps subgroup J avian leucosis virus enter cells

Lin Wang , Mei Mei , Aijian Qin , Jianqiang Ye , Kun Qian , Hongxia Shao

AbstractWe previously identified chicken Annexin A2 (chANXA2) as a novel receptor for retrovirus avian leucosis virus subgroup J (ALV-J), using a DF1 cell line expressing the viral envelope (env) protein. To further probe whether other proteins participate in virus infection, we investigated several host proteins from co-immunoprecipitation with the DF1 cell line expressing viral env. Mass spectrometry analysis indicates that the chicken glucose-regulation protein 78 (chGRP78) of the DF1 membrane interacted with the ALV-J env protein. The results revealed that antibodies or siRNA to chGRP78 significantly inhibited ALV-J infection and replication, and over-expression of chGRP78 enabled the entry of ALV-J into non-susceptible cells. Taken together, these results are the first to report that chGRP78 functions to help ALV-J enter cells.

Domaines: Sciences du Vivant [q-bio]

Lin Wang, Mei Mei, Aijian Qin, Jianqiang Ye, Kun Qian, et al.. Membrane-associated GRP78 helps subgroup J avian leucosis virus enter cells. Veterinary Research, 2016, 47 (1), pp.92. ⟨10.1186/s13567-016-0373-6⟩. ⟨hal-01390817⟩