Pulsatile microvascular blood flow imaging by short-time Fourier transform analysis of ultrafast laser holographic interferometry

Abstract : We report on wide-field imaging of pulsatile microvascular blood flow in the exposed cerebral cortex of a mouse by holographic interferometry. We recorded interferograms of laser light backscattered by the tissue, beating against an off-axis reference beam with a 50 kHz framerate camera. Videos of local Doppler contrasts were rendered numerically by Fresnel transformation and short-time Fourier transform analysis. This approach enabled instantaneous imaging of pulsatile blood flow contrasts in superficial blood vessels over 256 x 256 pixels with a spatial resolution of 10 microns and a temporal resolution of 20 ms.
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L. Puyo, I. Ferezou, A. Rancillac, M. Simonutti, M. Paques, et al.. Pulsatile microvascular blood flow imaging by short-time Fourier transform analysis of ultrafast laser holographic interferometry. 8th Biomedical Engineering International Conference(BMEiCON), IEEE Nov 2015, Pattaya, Thailand. pp.1 - 5, ⟨10.1109/BMEiCON.2015.7399525⟩. ⟨hal-01386362⟩

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