High speed optical holography of retinal blood flow

M. Pellizzari (1) , M. Simonutti (2) , J. Degardin (2) , José-Alain Sahel (2, 3) , M. Fink (1) , M. Paques (3, 2) , Michael Atlan (1)

1 IL - Institut Langevin - Ondes et Images (UMR7587)
2 Institut de la Vision
3 CHNO - Centre Hospitalier National d'Ophtalmologie des Quinze-Vingts

We performed non-invasive video imaging of retinal blood flow in a pigmented rat by holographic interferometry of near-infrared laser light backscattered by retinal tissue, beating against an off-axis reference beam sampled at a frame rate of 39 kHz with a high throughput camera. Local Doppler contrasts emerged from the envelopes of short-time Fourier transforms and the phase of autocorrelation functions of holograms rendered by Fresnel transformation. This approach permitted imaging of blood flow in large retinal vessels (30 microns diameter) over 400 by 400 pixels with a spatial resolution of 8 microns and a temporal resolution of 6.5 ms.

Mots clés: holography off-axis laser doppler high-speed

Domaines: Optique [physics.optics]

M. Pellizzari, M. Simonutti, J. Degardin, José-Alain Sahel, M. Fink, et al.. High speed optical holography of retinal blood flow. Optics Letters, 2016, 41 (15), pp.3503 - 3503. ⟨10.1364/OL.41.003503.v006⟩. ⟨hal-01386360⟩