OPTIMIZATION OF CULTURE CONDITIONS FOR FUNGAL GROWTH AND PRODUCTION OF RED PIGMENTS FROM TALAROMYCES albobiverticillius USING RESPONSE SURFACE METHODOLOGY

Mekala Venkatachalam (1, 2) , Alain Shum Cheong Sing (2) , Yanis Caro (2, 1) , Laurent Dufossé (2, 1) , Mireille Fouillaud (2, 1)

1 ESIROI Agroalimentaire, Saint-Denis, Reunion Island, Indian ocean, France
2 LCSNSA - Laboratoire de Chimie des Substances Naturelles et des Sciences des Aliments

Talaromyces albobiverticillius-is a pigment producing ascomycete fungi isolated from the marine biodiversity of Réunion Island, Indian Ocean, France. Due to the extensive interest in process development for pigment production from natural microbial sources among scientists and manufacturers, much attention has to be paid to unfold marine treasure to find some novel pigment producers. Media composition and growth conditions influence culture growth and thus have an effect on pigment production. The production of pigment(s) can be improved by optimizing the culture conditions. Traditional optimization techniques such as " One factor at a time " fail to identify the variables that give rise to maximum response, and also consume labor and time. Statistical methods are an alternate to traditional methods, a process by considering the mutual interactions among the variables to optimize responses. Response Surface Methodology (RSM) is one such technique which simplifies optimization study by considering mutual interactions in a statistically valid manner. A three level four factor Box Behnken Design (BBD) was used to identify the effect of process variables such as initial pH, temperature, agitation rate and incubation period on pigment production and biomass growth. From BBD results, the most optimal conditions for pigment production were found to be 24°C, 200rpm with initial pH of 6.5. The yield of pigment was measured in terms of OD values and then converted to amount of pigments in µg/l equivalent to the standards used. With these optimized conditions, further study will be carried out to observe the effects of different nutritional factors such as carbon, nitrogen sources, and trace elements on pigment production. Acknowledgements:

Mots clés: Talaromyces albobiverticillius; fungi; pigment; optimization; Response Surface Methodology

Domaines: Sciences du Vivant [q-bio]; Alimentation et Nutrition; Biotechnologies; Microbiologie et Parasitologie

Mekala Venkatachalam, Alain Shum Cheong Sing, Yanis Caro, Laurent Dufossé, Mireille Fouillaud. OPTIMIZATION OF CULTURE CONDITIONS FOR FUNGAL GROWTH AND PRODUCTION OF RED PIGMENTS FROM TALAROMYCES albobiverticillius USING RESPONSE SURFACE METHODOLOGY. Socaciu C.; Pintea A. International Congress of Pigments in Food – “Coloured foods for health benefits”, Jun 2016, Cluj-Napoca, Romania. Proceedings of the 8th International Congress of Pigments in Food – “Coloured foods for health benefits”, pp.55, International Congress of Pigments in Food – “Coloured foods for health benefits”. ⟨hal-01378058⟩