Colony development of laser printed eukaryotic (yeast and microalga) microorganisms in co-culture

Behnam Taidi (1) , Guillaume Lebernede (2) , Lothar Koch (3) , Patrick Perre (1) , Boris Chichkov (4, 3)

1 LGPM - Laboratoire de Génie des Procédés et Matériaux - EA 4038
2 Sup'Biotech
3 LZH - Laser Zentrum Hannover e.V.
4 Leibniz Universität Hannover=Leibniz University Hannover

Laser Induced Forward Transfer (LIFT) bioprinting is one of a group of techniques that have been largely applied for printing mammalian cells so far. Bioprinting allows precise placement of viable cells in a defined matrix with the aim of directed three-dimensional development of tissues. In this study, laser bioprinting is used to precisely place eukaryotic microorganisms in specific patterns that allow growth and microscopic observation of the organisms’ micro-colonies. Saccharomyces cerevisiae var. bayanus and Chlorella vulgaris are used as model organisms for this purpose. Growth and development of the micro-colonies are studied via confocal microscopy and the colonies’ growth rates are determined by image analysis. The developed protocols for printing of microorganisms and growth-rate deter-mination of the micro-colonies are very promising for future studies of colony growth and development.

Mots clés: growth rates of micro-colonies; printing of microorganisms; Saccharomyces cerevisiae; Chlorella vulgaris; laser-induced forward transfer; bioprinting

Domaines: Génie des procédés

Behnam Taidi, Guillaume Lebernede, Lothar Koch, Patrick Perre, Boris Chichkov. Colony development of laser printed eukaryotic (yeast and microalga) microorganisms in co-culture. International Journal of Bioprinting, 2016, 2 (2), pp.37-43. ⟨10.18063/IJB.2016.02.001⟩. ⟨hal-01372412⟩