Tolerance and Long-Term MRI Imaging of Gadolinium-Modified Meshes Used in Soft Organ Repair

Vincent Letouzey (1, 2) , Stéphanie Huberlant (1, 2) , Arnaud Cornille (1, 2) , Sébastien Blanquer (1) , Olivier Guillaume (1) , Laurent Lemaire (3) , Xavier Garric (1) , Renaud de Tayrac (2, 4)

1 IBMM - Institut des Biomolécules Max Mousseron [Pôle Chimie Balard]
2 CHU Nîmes - Centre Hospitalier Universitaire de Nîmes
3 MINT - Micro et Nanomédecines Biomimétiques
4 UM - Université de Montpellier

Background Synthetic meshes are frequently used to reinforce soft tissues. The aim of this translational study is to evaluate tolerance and long-term MRI visibility of two recently developed Gadolinium- modified meshes in a rat animal model. Materials and Methods Gadolinium-poly-ε-caprolactone (Gd-PCL) and Gadolinium-polymethylacrylate (Gd-PMA) modified meshes were implanted in Wistar rats and their tolerance was assessed daily. Inflammation and biocompatibility of the implants were assessed by histology and immunohistochemistry after 30 days post implantation. Implants were visualised by 7T and 3T MRI at day 30 and at day 90. Diffusion of Gadolinium in the tissues of the implanted animals was assessed by Inductively Coupled Plasma Mass Spectrometry. Results Overall Gd-PMA coated implants were better tolerated as compared to those coated with Gd-PCL. In fact, Gd-PMA implants were characterised by a high ratio collagen I/III and good vascularisation of the integration tissues. High resolution images of the coated mesh were obtained in vivo with experimental 7T as well as 3T clinical MRI. Mass spectrometry analyses showed that levels of Gadolinium in animals implanted with coated mesh were similar to those of the control group. Conclusions Meshes coated with Gd-PMA are better tolerated as compared to those coated with Gd- PCL as no signs of erosion or significant inflammation were detected at 30 days post implantation. Also, Gd-PMA coated meshes were clearly visualised with both 7T and 3T MRI devices. This new technique of mesh optimisation may represent a valuable tool in soft tissue repair and management.

Domaines: Biomatériaux

Vincent Letouzey, Stéphanie Huberlant, Arnaud Cornille, Sébastien Blanquer, Olivier Guillaume, et al.. Tolerance and Long-Term MRI Imaging of Gadolinium-Modified Meshes Used in Soft Organ Repair. PLoS ONE, 2015, ⟨10.1371/journal.pone.0120218⟩. ⟨hal-01369537⟩