The use of lysozyme to prepare biologically active chitooligomers

Abstract : Two types of crustacean commercial chitosans (CS1, CS2) were dissolved in lactic acid solutions, hydrolysed by lysozyme and finally fractioned by methanol solutions into two parts containing chito-oligomers (CS-O1, CS-O2). The antioxidant power and antimicrobial properties of both fractions were studied and compared with non-hydrolysed CS1 and CS2. The antioxidant properties were determined by the ferric ion reducing antioxidant power (FRAP) method while the bioactive properties were evaluated against a strain of Listeria monocytogenes. CS-O obtained from the solid fraction of the chito-oligomers solid fractions treated with 90% methanol showed the highest reducing power. Microbiological tests showed that CS-O exhibit higher antilisterial activity than CS.
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Submitted on : Wednesday, September 14, 2016 - 3:56:40 PM
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Anna Zimoch-Korzycka, Christian Gardrat, Alain Castellan, Véronique Coma, Andrzej Jarmoluk. The use of lysozyme to prepare biologically active chitooligomers . Polímeros, Associação Brasileira de Polímeros, 2015, 25 (1), pp.35-41. ⟨10.1590/0104-1428.1630⟩. ⟨hal-01366454⟩

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