Aflatoxin B1 detection using a highly-sensitive molecularly-imprinted electrochemical sensor based on an electropolymerized metal organic framework

Abstract : A sensitive electrochemical molecularly-imprinted sensor was developed for the detection of aflatoxin B1 (AFB1), by electropolymerization of p-aminothiophenol-functionalized gold nanoparticles in the presence of AFB1 as a template molecule. The extraction of the template leads to the formation of cavities that are able to specifically recognize and bind AFB1 through - interactions between AFB1 molecules and aniline moities. The performance of the developed sensor for the detection of AFB1 was investigated by linear sweep voltammetry using a hexacyanoferrate/hexacyanoferrite solution as a redox probe, the electron transfer rate increasing when the concentration of AFB1 increases, due to a p-doping effect. The molecularly-imprinted sensor exhibits a broad linear range, between 3.2 fM and 3.2 mu M, and a quantification limit of 3 fM. Compared to the non-imprinted sensor, the imprinting factor was found to be 10. Selectivity studies were also performed towards the binding of other aflatoxins and ochratoxin A, proving good selectivity.
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Toxins, MDPI, 2015, 7 (9), pp.3540-3553 〈10.3390/toxins7093540〉
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https://hal.archives-ouvertes.fr/hal-01363726
Contributeur : Agnès Bussy <>
Soumis le : dimanche 11 septembre 2016 - 14:14:41
Dernière modification le : mercredi 12 décembre 2018 - 15:38:06

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Mengjuan Jiang, Mohamed Braiek, Anca Florea, Amani Chrouda, Carole Farre, et al.. Aflatoxin B1 detection using a highly-sensitive molecularly-imprinted electrochemical sensor based on an electropolymerized metal organic framework . Toxins, MDPI, 2015, 7 (9), pp.3540-3553 〈10.3390/toxins7093540〉. 〈hal-01363726〉

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