An ultrasensitive nanobiohybrid platform for glucose electrochemical biosensing based on ferrocenyl iminopropyl-modified silica nanoparticles

Abstract : A novel nanobiohybrid platform based on ferrocenyl iminopropyl-modified silica nanoparticle conjugates (fap-SiNPs), entrapped in glucose oxidase (GOx) and bovine serum albumin cross-linked with glutaraldehyde, was developed. The as-synthesized nanobiohybrid composite was cast onto a paminothiophenol- modified gold electrode surface to form the platform for glucose electrochemical biosensing. Under optimal conditions, the device was able to detect glucose with a detection limit of 0.68 nM and a linear range from 1 nM to 100 mu M. The proximity of these three components: fap-SiNPs, aminothiophenol and GOx enhanced the electron-transfer between film and electrode. The applicability of the platform was tested on glucose detection in saliva. Furthermore, using HRP instead of GOx, the platform can be used to detect 1 pM of H2O2
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https://hal.archives-ouvertes.fr/hal-01363671
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Submitted on : Sunday, September 11, 2016 - 7:39:51 AM
Last modification on : Thursday, February 8, 2018 - 11:10:30 AM

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M Saadaoui, Mohamed Braiek, Nicole Jaffrezic-Renault, Noureddine Raouafi. An ultrasensitive nanobiohybrid platform for glucose electrochemical biosensing based on ferrocenyl iminopropyl-modified silica nanoparticles. RSC Advances, Royal Society of Chemistry, 2016, 6 (52), pp.46238-46243 ⟨10.1039/c6ra03779h⟩. ⟨hal-01363671⟩

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