Phytochemical and biological investigation of two diplotaxis species growing in Tunisia: D-virgata & D-erucoides

Abstract : A phytochemical investigation of Diplotaxis virgata D.C. and D. erucoides (L.) D.C. (Brassicaceae) offered to the isolation of two new flavonoids isorhamnetin-3-O--l-glucopyranoside (1) and rhamnetin-3,3'-di-O--d-glucopyranoside (2), respectively. Their structures have been elucidated from the extended spectroscopic methods, including 1D- and 2D-NMR, UV and mass spectrometry analysis and by comparison with literature data. The fatty acid composition of the hexane extracts of the two species was also investigated by using GC-MS. The antioxidant activity of ethanol, ethyl acetate, n-butanol extracts and the isolated compounds from the two species was evaluated using DPPH and ABTS(+) scavenging assays. All the tested samples showed an efficient radical scavenging ability, with IC50 values ranging from 16-40 mu g/mL for the DPPH and from 17-44 mu g/mL for the ABTS(+) assays. In addition, the antibacterial activity of the prepared extracts and compounds 1 and 2, determined by well diffusion agar method against two Gram positive and five Gram negative bacteria, was evaluated and the results showed significant effects against all strains used.
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Submitted on : Friday, August 26, 2016 - 4:37:31 PM
Last modification on : Saturday, March 30, 2019 - 1:33:04 AM

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Nizar Ben Salah, Hervé Casabianca, Hichem Ben Jannet, Sophie Chenavas, Sanglar Corinne, et al.. Phytochemical and biological investigation of two diplotaxis species growing in Tunisia: D-virgata & D-erucoides. Molecules, MDPI, 2015, 20 (10), pp.18128-18143. ⟨10.3390/molecules201018128⟩. ⟨hal-01356843⟩

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