Development and characterization of microsatellite markers for the oomyceta [i]Aphanomyces euteiches[/i].

Lucie Mieuzet (1) , Anne Quillevere Hamard (1) , Marie-Laure Pilet-Nayel (1) , Christophe Le May (1)

1 IGEPP - Institut de Génétique, Environnement et Protection des Plantes

Aphanomyces euteiches Drechsler is a serious pathogen of leguminous crops that causes devastating root rot of pea worldwide. Given that A. euteiches is a diploid organism, robust, codominant markers are needed for population genetics studies. We have developed and screened a microsatellite-enriched small-insert genomic library for identification of A. euteiches SSR containing sequences. Fourteen out of the 48 primer pairs designed to amplify SSR, produced unambiguous polymorphic products in our test population of 94 isolates. The number of alleles at each locus ranged from one to four. The identification of new markers would enhance the ability to evaluate the genetic structure of A. euteiches populations, and pathogen evolution.

Mots clés: SSR; Aphanomyces; Genetic study; Microsatellite; Oomyceta

Domaines: Biologie végétale

Lucie Mieuzet, Anne Quillevere Hamard, Marie-Laure Pilet-Nayel, Christophe Le May. Development and characterization of microsatellite markers for the oomyceta [i]Aphanomyces euteiches[/i].. Fungal Genetics and Biology, 2016, 91, pp.1-5. ⟨10.1016/j.fgb.2016.03.001⟩. ⟨hal-01345646⟩