Alternative Lengthening of Telomeres is characterized by reduced compaction of telomeric chromatin

Harikleia Episkopou (1) , Irena Draskovic (2, 3) , Amandine van Beneden (1) , Gaëlle Tilman (1) , Marina Mattiussi (1) , Matthieu Gobin (1) , Nausica Arnoult (1) , Arturo Londoño-Vallejo (3, 2) , Anabelle Decottignies (1)

1 UCL - Université Catholique de Louvain = Catholic University of Louvain
2 DIG CANCER - Dynamique de l'information génétique : bases fondamentales et cancer
3 UPMC - Université Pierre et Marie Curie - Paris 6

Proper telomeric chromatin configuration is thought to be essential for telomere homeostasis and stability. Previous studies in mouse suggested that loss of heterochromatin marks at telomeres might favor onset of Alternative Lengthening of Telomeres (ALT) pathway, by promoting homologous recombination. However, analysis of chromatin status at human ALT telomeres has never been reported. Here, using isogenic human cell lines and cellular hybrids, which rely either on telomerase or ALT to maintain telomeres, we show that chromatin com-paction is reduced at ALT telomeres and this is associated with a global decrease in telomeric H3K9me3. This, subsequently, leads to upregulation of telomere transcription. Accordingly, restoration of a more condensed telomeric chromatin through telomerase-dependent elongation of short ALT telomeres reduces telomere transcription. We further show that loss of ATRX chromatin remodeler function, a frequent characteristic of ALT cells, is not sufficient to decrease chromatin condensation at telomeres nor to increase the expression of telomeric RNA species. These results offer new insight on telomeric chromatin properties in ALT cells and support the hypothesis that telomeric chromatin decondensation is important for ALT pathway.

Domaines: Génétique; Cancer

Harikleia Episkopou, Irena Draskovic, Amandine van Beneden, Gaëlle Tilman, Marina Mattiussi, et al.. Alternative Lengthening of Telomeres is characterized by reduced compaction of telomeric chromatin. Nucleic Acids Research, 2014, 42 (7), pp.4391-4405. ⟨10.1093/nar/gku114⟩. ⟨hal-01332600⟩