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Article Dans Une Revue American Journal of Analytical Chemistry Année : 2014

Optimized Assay for Hydrogen Peroxide Determination in Plant Tissue Using Potassium Iodide

Résumé

Here, we present an optimization of colorimetric determination of hydrogen peroxide content in plants using potassium iodide. Our method is based on a one step buffer (extraction and reaction) for the determination of H 2 O 2 in different plant tissues and overcomes interference of soluble an-tioxidant and color background. A particular attention is paid to buffer pH shown to be tissue dependent. With this inexpensive microplate method, it is possible to analyze 12 experimental samples in about 45 min all in triplicates, with blanks, controls and standard curve.

Dates et versions

hal-01332406 , version 1 (15-06-2016)

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Sanders Junglee, Laurent Urban, Huguette Sallanon, Félicie Lopez-Lauri. Optimized Assay for Hydrogen Peroxide Determination in Plant Tissue Using Potassium Iodide. American Journal of Analytical Chemistry, 2014, ⟨10.4236/ajac.2014.511081⟩. ⟨hal-01332406⟩

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