Thin bacteria/Layered Double Hydroxide films using a layer-by-layer approach.

Matilte Halma (1) , Aicha Khenifi (2) , Martine Sancelme (1) , Pascale Pascale Besse, P. Besse-Hoggan Besse-Hoggan (1) , Pierre-Olivier Bussiere (1) , Vanessa Prévot (1) , Christine Mousty (1)

1 ICCF - Institut de Chimie de Clermont-Ferrand
2 Laboratoire de Physico-Chimie des Matériaux, Catalyse et Environnement Usto, El M’nouar

This paper reports the design of thin bacteria/Layered Double Hydroxides (LDH) films in which bacterial cells of Pseudomonas sp. strain ADP were assembled alternatively with Mg2Al-NO3 LDH nanosheets by a layer-by-layer deposition method. The UV–Vis spectroscopy was used to monitor the assembly process, showing a progressive increase in immobilized bacteria amount upon deposited cycles. The {ADP/LDH}n film was characterized by X-ray diffraction, infrared spectroscopy, scanning electron microscopy and atomic force microscopy. The metabolic activity of immobilized bacteria was determined using chronoamperometry by measuring the biochemical oxygen demand in presence of glucose using an artificial electron acceptor (Fe(CN)63−) at 0.5 V/Ag-AgCl. A steady current of 0.250 μA cm−2 was reached in about 30 s after the addition of 5 mM glucose.

Mots clés: Layered Double Hydroxides; Bacteria; Layer-by-layer; Thin film; Bioelectrode

Domaines: Chimie

Matilte Halma, Aicha Khenifi, Martine Sancelme, Pascale Pascale Besse, P. Besse-Hoggan Besse-Hoggan, Pierre-Olivier Bussiere, et al.. Thin bacteria/Layered Double Hydroxide films using a layer-by-layer approach.. Journal of Colloid and Interface Science, 2016, 474, pp.151-158. ⟨10.1016/j.jcis.2016.04.023⟩. ⟨hal-01324082⟩