Phototransformable fluorescent proteins: Future challenges.

Virgile Adam (1) , Romain Berardozzi (1) , Martin Byrdin (1) , Dominique Bourgeois (1)

1 IBS - UMR 5075 - Institut de biologie structurale

In fluorescence microscopy, the photophysical properties of the fluorescent markers play a fundamental role. The beauty of phototransformable fluorescent proteins (PTFPs) is that some of these properties can be precisely controlled by light. A wide range of PTFPs have been developed in recent years, including photoactivatable, photoconvertible and photoswitchable fluorescent proteins. These smart labels triggered a plethora of advanced fluorescence methods to scrutinize biological cells or organisms dynamically, quantitatively and with unprecedented resolution. Despite continuous improvements, PTFPs still suffer from limitations, and mechanistic questions remain as to how these proteins precisely work.

Mots clés: fluorescent proteins

Domaines: Biochimie, Biologie Moléculaire

Virgile Adam, Romain Berardozzi, Martin Byrdin, Dominique Bourgeois. Phototransformable fluorescent proteins: Future challenges.. Current Opinion in Chemical Biology, 2014, 20, pp.92-102. ⟨hal-01093360⟩