Improving the axial and lateral resolution of three-dimensional fluorescence microscopy using random speckle illuminations

Abstract : We consider a fluorescence microscope in which several three-dimensional images of a sample are recorded for different speckle illuminations. We show, on synthetic data, that by summing the positive deconvolution of each speckle image, one obtains a sample reconstruction with axial and transverse resolutions that compare favorably to that of an ideal confocal microscope.
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https://hal.archives-ouvertes.fr/hal-01314573
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Submitted on : Wednesday, May 11, 2016 - 3:52:25 PM
Last modification on : Friday, April 26, 2019 - 8:58:07 AM

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A. Negash, S. Labouesse, Nicolas Sandeau, Marc Allain, Hugues Giovannini, et al.. Improving the axial and lateral resolution of three-dimensional fluorescence microscopy using random speckle illuminations. Journal of the Optical Society of America. A, Optics and image science, Optical Society of America, 2016, 33, pp.1089-1094. ⟨10.1364/JOSAA.33.001089⟩. ⟨hal-01314573⟩

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