Leaf-mining by Phyllonorycter blancardella reprograms the host-leaf transcriptome to modulate phytohormones associated with nutrient mobilization and plant defense

Abstract : Phytohormones have long been hypothesized to play a key role in the interactions between plant-manipulating organisms and their host–plants such as insect–plant interactions that lead to gall or ‘green-islands’ induction. However, mechanistic understanding of how phytohormones operate in these plant reconfigurations is lacking due to limited information on the molecular and biochemical phytohormonal modulation following attack by plant-manipulating insects. In an attempt to fill this gap, the present study provides an extensive characterization of how the leaf-miner Phyllonorycter blancardella modulates the major phytohormones and the transcriptional activity of plant cells in leaves of Malus domestica.We show here, that cytokinins strongly accumulate in mined tissues despite a weak expression of plant cytokinin-related genes. Leaf-mining is also associated with enhanced biosynthesis of jasmonic acid precursors but not the active form, a weak alteration of the salicylic acid pathway and a clear inhibition of the abscisic acid pathway. Our study consolidates previous results suggesting that insects may produce and deliver cytokinins to the plant as a strategy to manipulate the physiology of the leaf to create a favorable nutritional environment. We also demonstrate that leaf-mining by P. blancardella leads to a strong reprogramming of the plant phytohormonal balance associated with increased nutrient mobilization, inhibition of leaf senescence and mitigation of plant direct and indirect defense
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Submitted on : Friday, May 20, 2016 - 12:23:35 PM
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Hui Zhang, Thomas Duge de Bernonville, Mélanie Body, Gaëlle Glevarec, Michael Reichelt, et al.. Leaf-mining by Phyllonorycter blancardella reprograms the host-leaf transcriptome to modulate phytohormones associated with nutrient mobilization and plant defense. Journal of Insect Physiology, Elsevier, 2016, Plant-reprogramming insects: from effector molecules to ecosystem engineering, 84, pp.114-127. ⟨10.1016/j.jinsphys.2015.06.003⟩. ⟨hal-01313546⟩

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