Skip to Main content Skip to Navigation
Journal articles

Nonsense-Mediated Decay Restricts LncRNA Levels in Yeast Unless Blocked by Double-Stranded RNA Structure

Abstract : Antisense long non-coding (aslnc)RNAs represent a substantial part of eukaryotic transcriptomes that are, in yeast, controlled by the Xrn1 exonuclease. Nonsense-Mediated Decay (NMD) destabilizes the Xrn1-sensitive aslncRNAs (XUT), but what determines their sensitivity remains unclear. We report that 3′ single-stranded (3′-ss) extension mediates XUTs degradation by NMD, assisted by the Mtr4 and Dbp2 helicases. Single-gene investigation, genome-wide RNA analyses, and double-stranded (ds)RNA mapping revealed that 3′-ss extensions discriminate the NMD-targeted XUTs from stable lncRNAs. Ribosome profiling showed that XUT are translated, locking them for NMD activity. Interestingly, mutants of the Mtr4 and Dbp2 helicases accumulated XUTs, suggesting that dsRNA unwinding is a critical step for degradation. Indeed, expression of anticomplementary transcripts protects cryptic intergenic lncRNAs from NMD. Our results indicate that aslncRNAs form dsRNA that are only translated and targeted to NMD if dissociated by Mtr4 and Dbp2. We propose that NMD buffers genome expression by discarding pervasive regulatory transcripts.
Document type :
Journal articles
Complete list of metadatas

Cited literature [48 references]  Display  Hide  Download
Contributor : Gilles d'Eggis <>
Submitted on : Wednesday, May 4, 2016 - 1:08:22 PM
Last modification on : Wednesday, December 9, 2020 - 3:12:53 PM
Long-term archiving on: : Tuesday, November 15, 2016 - 8:05:36 PM


Publication funded by an institution



Maxime Wery, Marc Descrimes, Nicolas Vogt, Anne-Sophie Dallongeville, Daniel Gautheret, et al.. Nonsense-Mediated Decay Restricts LncRNA Levels in Yeast Unless Blocked by Double-Stranded RNA Structure. Molecular & Cellular Oncology, Taylor et Francis, 2016, 61 (3), pp.379-392. ⟨10.1016/j.molcel.2015.12.020⟩. ⟨hal-01311517⟩



Record views


Files downloads