Channel characteristics of VDAC-3 from Arabidopsis thaliana.

Catherine Berrier (1) , Rémi Peyronnet (2) , Jean-Michel Betton (3) , Genevieve Ephritikhine (2) , Hélène Barbier-Brygoo (2) , Jean-Marie Frachisse (2) , Alexandre Ghazi (1)

1 NeuroPSI - Institut des Neurosciences Paris-Saclay
2 I2BC - Institut de Biologie Intégrative de la Cellule
3 Microb. Struc. (UMR_3528 / U-Pasteur_5) - Microbiologie structurale - Structural Microbiology

Four different isoforms of the Voltage-Dependent Anion Channel (VDAC) have been identified in Arabidopsis plant cells. The electrophysiological characteristics of several VDAC channels from animal as well as plant cells are well documented, but those of this model plant are unknown. One isoform, AtVDAC-3 was obtained either directly by cell-free synthesis or produced in Escherichia coli, as inclusion bodies, and re-natured. An electrophysiological study of the purified proteins in planar lipid bilayers showed that both methods yielded proteins with similar channel activity. The characteristics of AtVDAC-3 are that of a bona fide VDAC-like channel.

Domaines: Neurobiologie; Psychologie et comportements; Sciences cognitives

Catherine Berrier, Rémi Peyronnet, Jean-Michel Betton, Genevieve Ephritikhine, Hélène Barbier-Brygoo, et al.. Channel characteristics of VDAC-3 from Arabidopsis thaliana.. Biochemical and Biophysical Research Communications, 2015, 459 (1), pp.24-8. ⟨10.1016/j.bbrc.2015.02.034⟩. ⟨hal-01306719⟩