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Asymmetric Preorganization of Inverted Pair Residues in the Sodium-Calcium Exchanger

Abstract : In analogy with many other proteins, Na(+)/Ca(2+) exchangers (NCX) adapt an inverted twofold symmetry of repeated structural elements, while exhibiting a functional asymmetry by stabilizing an outward-facing conformation. Here, structure-based mutant analyses of the Methanococcus jannaschii Na(+)/Ca(2+) exchanger (NCX_Mj) were performed in conjunction with HDX-MS (hydrogen/deuterium exchange mass spectrometry) to identify the structure-dynamic determinants of functional asymmetry. HDX-MS identified hallmark differences in backbone dynamics at ion-coordinating residues of apo-NCX_Mj, whereas Na(+)or Ca(2+) binding to the respective sites induced relatively small, but specific, changes in backbone dynamics. Mutant analysis identified ion-coordinating residues affecting the catalytic capacity (kcat/Km), but not the stability of the outward-facing conformation. In contrast, distinct "noncatalytic" residues (adjacent to the ion-coordinating residues) control the stability of the outward-facing conformation, but not the catalytic capacity. The helix-breaking signature sequences (GTSLPE) on the α1 and α2 repeats (at the ion-binding core) differ in their folding/unfolding dynamics, while providing asymmetric contributions to transport activities. The present data strongly support the idea that asymmetric preorganization of the ligand-free ion-pocket predefines catalytic reorganization of ion-bound residues, where secondary interactions with adjacent residues couple the alternating access. These findings provide a structure-dynamic basis for ion-coupled alternating access in NCX and similar proteins.
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https://hal.archives-ouvertes.fr/hal-01299020
Contributor : Frank Thomas <>
Submitted on : Thursday, April 7, 2016 - 9:06:06 AM
Last modification on : Tuesday, January 19, 2021 - 10:46:15 AM

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Moshe Giladi, Lior Almagor, Liat van Dijk, Reuben Hiller, Petr Man, et al.. Asymmetric Preorganization of Inverted Pair Residues in the Sodium-Calcium Exchanger. Scientific Reports, Nature Publishing Group, 2016, 6, ⟨10.1038/srep20753⟩. ⟨hal-01299020⟩

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