3D printed PEGDA microstructures for gelatin scaffold integration and neuron differentiation

Xiaolong Tu (1) , Lina Wang (2) , Wei Jin (1) , Bin Wang (1) , Yadong Tang (1) , Jian Shi (1) , Zhijun Zhang (2) , Yong Chen (3, 4, 5)

1 PASTEUR - Processus d'Activation Sélective par Transfert d'Energie Uni-électronique ou Radiatif (UMR 8640)
2 CAAS - Chinese Academy of Agricultural Sciences
3 Institute for Interdisciplinary Research
4 PASTEUR - Processus d'Activation Sélective par Transfert d'Energie Uni-électronique ou Radiatif (UMR 8640)
5 Institute for Integrated Cell-Material Sciences

Three dimensional (3D) printing techniques can be used for scaffold fabrication but the most of them are limited by resolution and material choice. To bypass these limitations, we developed an approach by combining conventional 3D printing and freeze-drying techniques to produce lattice-type backbone and embedding microporous structures. Polyethylene glycol diacrylate (PEGDA), a biocompatible and photosensitive pre-polymer, was chosen for 3D printing of the backbone, while gelatin was used for the formation of microporous structures. The fabricated PEGDA/gelatin scaffolds were used for culture and differentiation of neural progenitor cells (NPCs), showing infiltration of the cells and outgrowth of differentiated neurites. This strategy of combined use of 3D printing and freeze-drying techniques might be useful for scaffold fabrication in terms of easy design and easy material processing.

Mots clés: 3D printing; PEGDA; Gelatin; Microstructure; Neuron differentiation

Domaines: Micro et nanotechnologies/Microélectronique

Xiaolong Tu, Lina Wang, Wei Jin, Bin Wang, Yadong Tang, et al.. 3D printed PEGDA microstructures for gelatin scaffold integration and neuron differentiation. Microelectronic Engineering, 2016, ⟨10.1016/j.mee.2016.03.007⟩. ⟨hal-01285501⟩