Skip to Main content Skip to Navigation
Journal articles

Characterization and Multiplexing of 21 Microsatellite Markers for the Herb Noccaea caerulescens (Brassicaceae)

Abstract : Premise of the study: Multiplexed microsatellite markers were developed for population genetic studies in the pseudometallophyte Noccaea caerulescens (Brassicaceae), a model species to investigate metal tolerance and hyperaccumulation in higher plants. • Methods and Results: Microsatellite loci were isolated through pyrosequencing of an enriched DNA library. Three multiplexes combining four previously published and 17 newly designed markers were developed. The new markers were screened in metallicolous and nonmetallicolous populations from southern France. The total number of alleles per locus ranged from fi ve to 18. The observed heterozygosity per locus and per population ranged from 0 to 0.83, and expected heterozygosity ranged from 0 to 0.89. • Conclusions: The investigated loci showed reasonable to high levels of polymorphism at the regional scale. The multiplex set should be helpful in investigating genetic diversity, population structure, and demographic history in N. caerulescens at various spatial scales.
Complete list of metadatas

Cited literature [15 references]  Display  Hide  Download

https://hal.archives-ouvertes.fr/hal-01282559
Contributor : Archive Ouverte Prodinra <>
Submitted on : Thursday, March 3, 2016 - 8:17:48 PM
Last modification on : Thursday, May 28, 2020 - 11:18:44 AM
Document(s) archivé(s) le : Saturday, June 4, 2016 - 11:30:44 AM

File

Gonneau2015_{345EA823-56EA-424...
Explicit agreement for this submission

Identifiers

Collections

Citation

Mathilde Mousset, Elodie Flaven, Fabienne Justy, Juliette Pouzadoux, Cécile Gode, et al.. Characterization and Multiplexing of 21 Microsatellite Markers for the Herb Noccaea caerulescens (Brassicaceae). Applications in Plant Sciences, Wiley, 2015, 3 (12), pp.1500052. ⟨10.3732/apps.1500052⟩. ⟨hal-01282559⟩

Share

Metrics

Record views

158

Files downloads

166