Characterization and Multiplexing of 21 Microsatellite Markers for the Herb Noccaea caerulescens (Brassicaceae)

Mathilde Mousset (1, 2) , Elodie Flaven (1, 2) , Fabienne Justy (1, 2) , Juliette Pouzadoux (1, 2) , Cécile Gode (3) , Maxime Pauwels (3) , Cédric Gonneau (4)

1 EPHE - École Pratique des Hautes Études
2 UMR ISEM - Institut des Sciences de l'Evolution de Montpellier
3 Evo-Eco-Paléo (EEP) - Évolution, Écologie et Paléontologie (Evo-Eco-Paleo) - UMR 8198
4 LSE - Laboratoire Sols et Environnement

Premise of the study: Multiplexed microsatellite markers were developed for population genetic studies in the pseudometallophyte Noccaea caerulescens (Brassicaceae), a model species to investigate metal tolerance and hyperaccumulation in higher plants. • Methods and Results: Microsatellite loci were isolated through pyrosequencing of an enriched DNA library. Three multiplexes combining four previously published and 17 newly designed markers were developed. The new markers were screened in metallicolous and nonmetallicolous populations from southern France. The total number of alleles per locus ranged from fi ve to 18. The observed heterozygosity per locus and per population ranged from 0 to 0.83, and expected heterozygosity ranged from 0 to 0.89. • Conclusions: The investigated loci showed reasonable to high levels of polymorphism at the regional scale. The multiplex set should be helpful in investigating genetic diversity, population structure, and demographic history in N. caerulescens at various spatial scales.

Mots clés: pseudometallophyte; Noccaea caerulescens; Heavy metal tolerance; microsatellite; Brassicaceae

Domaines: Sciences du Vivant [q-bio]

Mathilde Mousset, Elodie Flaven, Fabienne Justy, Juliette Pouzadoux, Cécile Gode, et al.. Characterization and Multiplexing of 21 Microsatellite Markers for the Herb Noccaea caerulescens (Brassicaceae). Applications in Plant Sciences, 2015, 3 (12), pp.1500052. ⟨10.3732/apps.1500052⟩. ⟨hal-01282559⟩