HAL will be down for maintenance from Friday, June 10 at 4pm through Monday, June 13 at 9am. More information
Skip to Main content Skip to Navigation
Journal articles

Effects of 60-GHz Millimeter Waves on Neurite Outgrowth in PC12Cells Using High Content Screening

Abstract : Technologies for wireless telecommunication systems using millimeter waves (MMW) will be widely deployed in the near future. Forthcoming applications in this band, especially around 60 GHz, are mainly developed for high data-rate local and body-centric telecommunications. At those frequencies, electromagnetic radiations have a very shallow penetration into biological tissues, making skin keratinocytes, and free nerve endings of the upper dermis the main targets of MMW. Only a few studies assessed the impact of MMW on neuronal cells, and none of them investigated a possible effect on neuronal differentiation. We used a neuron-like cell line (PC12), which undergoes neuronal differentiation when treated with the neuronal growth factor (NGF). PC12 cells were exposed at 60.4 GHz for 24 h, at an incident power density averaged over the cell monolayer of 10 mW/cm2. Using a large scale cell-by-cell analysis based on high-content screening microscopy approach, we assessed potential effects of MMW on PC12 neurite outgrowth and cytoskeleton protein expression. No differences were found in protein expression of the neuronal marker β3-Tubulin nor in internal expression control β-Tubulin. On the other hand, our data showed a slight increase, although insignificant, in neurite outgrowth, induced by MMW exposure. However, experimental controls demonstrated that this increase was related to heating
Document type :
Journal articles
Complete list of metadata

Cited literature [37 references]  Display  Hide  Download

Contributor : Nelly Brenner Connect in order to contact the contributor
Submitted on : Tuesday, April 26, 2016 - 4:48:10 PM
Last modification on : Wednesday, April 27, 2022 - 3:50:38 AM


Effects of 60-GHz Millimeter W...
Files produced by the author(s)



Alexis Haas, Yann Le Page, Maxim Zhadobov, Ronan Sauleau, Yves Le Dréan. Effects of 60-GHz Millimeter Waves on Neurite Outgrowth in PC12Cells Using High Content Screening. Neuroscience Letters, Elsevier, 2016, 618, pp.58-65. ⟨10.1016/j.neulet.2016.02.038⟩. ⟨hal-01281481⟩



Record views


Files downloads