Hapten Synthesis for a Monoclonal Antibody Based ELISA for Deltamethrin

Abstract : An enzyme-linked immunosorbent assay (ELISA) was developed for the insecticide deltamethrin. Two haptens were synthesized:  the first one was a derivative of the whole molecule with a spacer arm bound to the aromatic ring; the second one was a derivative of deltamethric acid. Twelve monoclonal antibodies were obtained. A competitive ELISA using monoclonal antibody Del 01 yielded the most sensitive assay. The IC50 value for deltamethrin was estimated to be 0.5 μg mL-1 with a detection limit of 0.08 μg mL-1. Monoclonal antibody Del 01 seems to be deltamethrin specific since <1% cross-reactivity with other pyrethroid analogues was observed.
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Anne-Laurence Queffelec, Patrice Nodet, Jean-Pierre Haelters, Daniel Thouvenot, Bernard Corbel. Hapten Synthesis for a Monoclonal Antibody Based ELISA for Deltamethrin. Journal of Agricultural and Food Chemistry, American Chemical Society, 1998, 46 (4), pp.1670-1676. ⟨10.1021/jf9703922⟩. ⟨hal-01274840⟩



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