Immuno-enzymatic staining procedure for Pythium species with filamentous non-inflated sporangia in soilless cultures

Abstract : Polyclonal antibodies were prepared against crude cell wall fractions of a Pythium isolate with filamentous non-inflated sporangia, associated with root rot of tomato in soilless cultures. In ELISA tests, the antiserum showed sensitivity and specificity against original antigens and those from closely related isolates with filamentous sporangia (such as Pythium aquatile and Pythium coloratum). The antiserum reacted weakly with mycelial preparations of several fungi commonly found on tomato roots, such as Fusarium spp. or Penicillium spp. An immunocytochemical staining test was developed in order to study the colonization by filamentous non-inflated sporangial isolates of Pythium in the presence of other fungi in soilless cultures.
Keywords : Pythium
Complete list of metadatas

https://hal.archives-ouvertes.fr/hal-01274814
Contributor : Patrice Nodet <>
Submitted on : Tuesday, February 16, 2016 - 11:32:25 AM
Last modification on : Thursday, November 29, 2018 - 4:08:55 PM

Links full text

Identifiers

Collections

Citation

Catherine Rafin, Patrice Nodet, Yves Tirilly. Immuno-enzymatic staining procedure for Pythium species with filamentous non-inflated sporangia in soilless cultures. Mycological Research, Elsevier, 1994, 98 (5), pp.535-541. ⟨10.1016/S0953-7562(09)80475-9⟩. ⟨hal-01274814⟩

Share

Metrics

Record views

87